Expression of the ferrioxamine receptor gene of Erwinia amylovora CFBP 1430 during pathogenesis

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A Dellagi1, D Reis, B Vian

  • 1Laboratoire de Pathologie Végétale, INRA/INA P-G, Paris, France.

Molecular Plant-Microbe Interactions : MPMI
|May 5, 1999
PubMed

Insights

Erwinia amylovora requires iron for fire blight. Its ferrioxamine receptor gene (foxR) is activated under iron-restricted conditions and within apple leaves, crucial for disease progression.

Area of Science:

  • Plant Pathology
  • Microbial Pathogenesis
  • Molecular Biology

Background:

  • Erwinia amylovora causes fire blight, a devastating disease in apple and pear trees.
  • Iron acquisition is critical for bacterial virulence, and high-affinity transport systems are often essential for plant pathogens.

Purpose of the Study:

  • To investigate the in planta expression of the Erwinia amylovora ferrioxamine receptor gene (foxR).
  • To understand the role of iron transport in the pathogenesis of fire blight disease.

Main Methods:

  • Construction of a chromosomal transcriptional lacZ fusion in E. amylovora CFBP 1430.
  • Analysis of lacZ expression in planta under varying iron conditions.
  • Microscopic examination of gene expression relative to bacterial localization and density.

Main Results:

  • The foxR gene showed high induction under iron-restricted conditions.
  • LacZ activity was significantly elevated in inoculated apple leaf tissues.
  • Differential gene expression correlated with bacterial cell localization and density within diseased tissues.

Conclusions:

  • The ferrioxamine receptor gene (foxR) is actively expressed by E. amylovora during infection in apple leaves.
  • Regulation of iron transport systems in response to environmental cues is vital for fire blight disease development.
  • Targeting iron acquisition mechanisms could be a strategy to control fire blight.

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