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Sonodynamically induced cell damage with 4'-O-tetrahydropyranyladriamycin, THP
N Yumita1, M Kaneuchi, Y Okano
1School of Pharmaceutical Sciences, Toho University, Chiba, Japan.
Background:
4'-O-tetrahydropyranyladriamycin (THP) is a novel anthracycline derivative with cardiotoxicity significantly lower than ADM. In this study, the ultrasonically induced in vitro cell damaging effect of THP was investigated.
Materials And Methods:
Sarcoma 180 cells suspended in air-saturated PBS were exposed to ultrasound for up to 60 s in the presence and absence of THP. The viability of the isolated cells was determined by staining of the cells with Trypan Blue dye.
Results:
The rate of inducing cell damage with ultrasound was doubled with 80 microM THP, while no cell damage was observed with THP alone.
Conclusion:
The enhancement of ultrasonically induced in vitro cell damage was demonstrated with THP. This enhancement was significantly inhibited by histidine, which may suggest a sonochemical mechanism.