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Sequence analysis of F, SH, and HN genes among mumps virus strains in Japan

Y Kashiwagi1, T Takami, T Mori

  • 1Department of Pediatrics, Tokyo Medical University, Japan.

Archives of Virology
|May 5, 1999
PubMed

Insights

Mumps virus (MuV) genetic sequencing reveals two main genotypes, A and B, in Japan since 1976. Indigenous Japanese lineages (A1-A3) differ from genotype B, which clusters with strains from Europe and North America.

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Mumps virus (MuV) poses a significant public health concern.
  • Understanding MuV genetic diversity is crucial for effective control strategies.
  • Previous studies have not fully elucidated the genetic evolution of MuV in Japan.

Purpose of the Study:

  • To analyze the genetic variability of mumps virus strains in Japan.
  • To determine the evolutionary relationships of Japanese MuV strains.
  • To investigate the origins and spread of different MuV genotypes.

Main Methods:

  • Sequencing of fusion (F), small hydrophobic (SH), and hemagglutinin-neuraminidase (HN) genes from 15 MuV strains.
  • Phylogenetic analysis to classify MuV strains into genotypes and subtypes.
  • Comparative analysis of Japanese MuV strains with international strains.

Main Results:

  • Mumps virus strains in Japan were classified into two major genotypes: A and B.
  • Genotype A was further subdivided into three subtypes: A1, A2, and A3.
  • Genotypes A1, A2, and A3 represent indigenous Japanese lineages, while genotype B clusters with European and North American strains.

Conclusions:

  • Mumps virus in Japan has evolved into distinct indigenous lineages.
  • Genotype B strains suggest potential introduction events from Europe and North America.
  • Continued genetic surveillance is essential for monitoring MuV evolution and informing public health interventions.

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