Comparison of the electrooptical properties and specific respiratory activity of Acinetobacter calcoaceticum A-122
O V Ignatov1, N A Khorkina, Shchyogolev SYu
1Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia. oignatov@ibppm.saratov.su
Abstract:
The electrooptical (EO) properties of a cell suspension and the specific respiratory activity of cells towards p-nitrophenol (PNP) were compared during PNP metabolism in Acinetobacter calcoaceticum strain A-122. The frequency dependence of the suspension's turbidity changes due to cellular orientation (orientational spectra) at frequencies of an orienting electric field of 10-10,000 kHz was determined. Orientational spectral changes observed during PNP incubation of the cells were followed over the range of 10-502 kHz. There were linear relationships between the magnitude of the EO effect at a 502-kHz frequency and the concentration of PNP over the range of 0.1-0.8 mM, and between the specific respiratory activity of the cells and the concentration of PNP over the range of 0.1-1.0 mM. The knowledge gained from these studies suggests a direct relationship between alterations in the cellular EO properties and PNP metabolism.
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