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Comparison of the electrooptical properties and specific respiratory activity of Acinetobacter calcoaceticum A-122.

O V Ignatov¹, N A Khorkina, Shchyogolev SYu

¹Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia. oignatov@ibppm.saratov.su

FEMS Microbiology Letters

|May 5, 1999

Summary

This summary is machine-generated.

Electrooptical (EO) properties of Acinetobacter calcoaceticum cells correlate with p-nitrophenol (PNP) metabolism. Changes in EO properties during PNP degradation indicate a direct relationship with cellular respiratory activity.

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Area of Science:

Microbiology
Biophysics

Background:

Acinetobacter calcoaceticum metabolizes p-nitrophenol (PNP).
Electrooptical (EO) properties reflect cellular characteristics.

Purpose of the Study:

To investigate the relationship between EO properties and PNP metabolism in Acinetobacter calcoaceticum.
To determine if EO changes can monitor cellular respiratory activity during PNP degradation.

Main Methods:

Measuring frequency-dependent orientational spectra of cell suspensions in an electric field (10-10,000 kHz).
Assessing specific respiratory activity towards PNP.
Correlating EO spectral changes with PNP concentration and respiratory activity.

Main Results:

Orientational spectral changes were observed during PNP incubation within the 10-502 kHz range.
A linear relationship was found between the EO effect magnitude (at 502 kHz) and PNP concentration (0.1-0.8 mM).
A linear relationship was also observed between specific respiratory activity and PNP concentration (0.1-1.0 mM).

Conclusions:

Alterations in cellular EO properties are directly related to PNP metabolism.
EO properties can serve as an indicator of cellular respiratory activity during PNP degradation.