Effect of sterilization on the physical and structural characteristics of polyhydroxyoctanoate (PHO)

The present study examined the potential applicability of poly(beta-hydroxy octanoate) (PHO), a bacterial polyester, as a candidate for biomaterial applications, by investigating the effect of sterilization on the physical and structural characteristics of PHO. PHO-cast films were sterilized by either ethylene oxide (EO) gas at 38 degrees C or gamma radiation (2.5 Mrad) in air at room temperature. The physical characteristics of the EO and gamma-sterilized PHO were determined by scanning electron microscopy (SEM) and tensile strength analyses. In addition, various analytical methods were used to detect modifications in the chemical and morphological structure of PHO, namely, electron spectroscopy for chemical analysis (ESCA), Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), wide angle X-ray diffraction (WAXD), and size exclusion chromatography (SEC). The results show that EO sterilization did not modify the chemical and physical characteristics of PHO, however, significant modifications in both the structural and tensile properties were observed with gamma-sterilized PHO. These changes accounted for decreases in both the weight average, number average and melting temperature, and increases in the heat of fusion and tensile strength. No residual EO was detected following sterilization as revealed by head-space chromatography. The physical and structural properties of PHO were shown to be well preserved following EO sterilization, whereas gamma radiation caused random chain scission and physical cross-linking, a frequent phenomenon observed with organic polymers.