Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrophilic Aromatic Substitution: Fluorination and Iodination of Benzene01:13

Electrophilic Aromatic Substitution: Fluorination and Iodination of Benzene

Bromination and chlorination of aromatic rings by electrophilic aromatic substitution reactions are easily achieved, but fluorination and iodination are difficult to achieve. Fluorine is so reactive that its reaction with benzene is difficult to control, resulting in poor yields of monofluoroaromatic products. To address this, Selectfluor reagent is used as a fluorine source in which a fluorine atom is bonded to a positively charged nitrogen.
Antipsychotic Drugs: Typical and Atypical Agents01:21

Antipsychotic Drugs: Typical and Atypical Agents

Antipsychotic drugs are classified into first-generation (typical) drugs including phenothiazines; and second-generation (atypical) drugs. Chlorpromazine hydrochloride (Thorazine), a phenothiazine derivative, broadly impacts the central, autonomic, and endocrine systems. This drug, along with typical agents like haloperidol (Haldol), primarily works by antagonizing D2 receptors, thus reducing dopaminergic neurotransmission. However, typical antipsychotics can cause side effects such as sedation...
Chemotherapy-Induced Nausea and Vomiting: 5-HT3 Receptor Antagonists01:27

Chemotherapy-Induced Nausea and Vomiting: 5-HT3 Receptor Antagonists

5-HT3 receptor antagonists, such as dolasetron, granisetron (Kytril), ondansetron (Zofran), and palonosetron (Axoli), are crucial in managing chemotherapy-induced nausea and vomiting (CINV) and postoperative nausea. These drugs selectively block 5-HT3 receptors in the visceral vagal and spinal afferent nerves, chemoreceptor trigger zone, and the vomiting center. They have a rapid onset of action and can be given as a single dose before chemotherapy. Ondansetron and granisetron, in particular,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fluoro analogs of WAY-100635 with varying pharmacokinetics properties.

Nuclear medicine and biology·2000
Same author

Measurement of dopamine release with continuous infusion of [11C]raclopride: optimization and signal-to-noise considerations.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2000
Same author

The suitability of [11C]-alpha-methyl-L-tryptophan as a tracer for serotonin synthesis: studies with dual administration of [11C] and [14C] labeled tracer.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism·2000
Same author

Radiotracer-derived biochemical profiles via a mathematical model: implications for therapeutic intervention.

Nuclear medicine and biology·1998
Same author

Brain serotonin synthesis rates in rhesus monkeys determined by [11C]alpha-methyl-L-tryptophan and positron emission tomography compared to CSF 5-hydroxyindole-3-acetic acid concentrations.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·1998
Same author

Defective actin polymerization in EBV-transformed B-cell lines from patients with the Wiskott-Aldrich syndrome.

The Journal of pathology·1998

Related Experiment Video

Updated: Jul 15, 2026

Microwave-assisted One-pot Synthesis of N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB)
08:33

Microwave-assisted One-pot Synthesis of N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB)

Published on: June 28, 2011

Development of fluorine-18-labeled 5-HT1A antagonists.

L Lang1, E Jagoda, B Schmall

  • 1Positron Emission Tomography Department, Clinical Center, National Institutes of Health, 10 Center Drive, Bethesda, Maryland 20892, USA.

Journal of Medicinal Chemistry
|May 7, 1999
PubMed
Summary

Researchers developed five fluorinated derivatives of WAY 100635 for serotonin receptor imaging. [18F]4d showed comparable properties to [11C]4a, while [18F]4c is suitable for dynamic serotonin level assessment.

More Related Videos

18F-Labeling of Radiotracers Functionalized with a Silicon Fluoride Acceptor (SiFA) for Positron Emission Tomography
09:57

18F-Labeling of Radiotracers Functionalized with a Silicon Fluoride Acceptor (SiFA) for Positron Emission Tomography

Published on: January 11, 2020

Radiosynthesis of 1-(2-[18F]Fluoroethyl)-L-Tryptophan using a One-pot, Two-step Protocol
08:33

Radiosynthesis of 1-(2-[18F]Fluoroethyl)-L-Tryptophan using a One-pot, Two-step Protocol

Published on: September 21, 2021

Related Experiment Videos

Last Updated: Jul 15, 2026

Microwave-assisted One-pot Synthesis of N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB)
08:33

Microwave-assisted One-pot Synthesis of N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB)

Published on: June 28, 2011

18F-Labeling of Radiotracers Functionalized with a Silicon Fluoride Acceptor (SiFA) for Positron Emission Tomography
09:57

18F-Labeling of Radiotracers Functionalized with a Silicon Fluoride Acceptor (SiFA) for Positron Emission Tomography

Published on: January 11, 2020

Radiosynthesis of 1-(2-[18F]Fluoroethyl)-L-Tryptophan using a One-pot, Two-step Protocol
08:33

Radiosynthesis of 1-(2-[18F]Fluoroethyl)-L-Tryptophan using a One-pot, Two-step Protocol

Published on: September 21, 2021

Area of Science:

  • Radiochemistry
  • Neuroscience
  • Pharmacology

Background:

  • WAY 100635 is a selective serotonin 5-HT1A receptor antagonist.
  • Development of novel radiotracers for 5-HT1A receptor imaging is crucial for neurological research.
  • Fluorine-18 (F-18) labeled tracers offer advantages in positron emission tomography (PET) imaging due to their favorable decay characteristics.

Purpose of the Study:

  • To synthesize and evaluate novel fluorinated derivatives of WAY 100635 labeled with F-18.
  • To compare the pharmacokinetic and binding properties of these new tracers with the established [carbonyl-11C]WAY 100635.
  • To determine the suitability of these F-18 tracers for static and dynamic imaging of 5-HT1A receptors.

Main Methods:

  • Synthesis of five fluorinated WAY 100635 derivatives using various fluorinated acids.
  • Radiolabeling of the derivatives with F-18.
  • In vivo evaluation in rats, including biodistribution, metabolite analysis, and receptor binding studies using quantitative autoradiography.

Main Results:

  • [18F]4d (FCWAY) demonstrated comparable brain kinetics and specific binding to [carbonyl-11C]WAY 100635, with faster blood clearance.
  • [18F]4b (FBWAY) and [18F]4c (MeFBWAY) showed rapid brain efflux and lower specific binding ratios.
  • A strong correlation (R > 0.97) was observed between in vivo differential uptake ratio (DUR) and in vitro receptor concentration across all compounds.

Conclusions:

  • [18F]4d is a promising F-18 tracer for static 5-HT1A receptor quantification due to its high contrast and favorable properties.
  • [18F]4c shows potential for assessing dynamic changes in serotonin levels.
  • These novel F-18 labeled WAY 100635 derivatives offer valuable tools for 5-HT1A receptor research.