Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA replication and head assembly in bacteriophage T4.

V M Andreev, E M Gonikberg

    Molekuliarnaia Biologiia
    |July 1, 1976
    PubMed
    Summary

    Bacteriophage T4 morphogenesis can occur independently of phage DNA replication. This study demonstrates that essential phage components assemble even when DNA synthesis is inhibited, revealing a novel aspect of viral assembly.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Photosensitizing properties of 3,3'-diethylthiacarbocyanine in biological media].

    Radiatsionnaia biologiia, radioecologiia·2015
    Same author

    [Application of the complex of DNA with the congo red anionic diazo dye for detection of nuclease-producing colonies of marine bacteria].

    Mikrobiologiia·2007
    Same author

    [A novel method of transfection of marine bacteria Pseudoalteromonas espejiana with deoxyribonucleic acid of bacteriophage PM2].

    Genetika·2006
    Same author

    [Comparative study of cytosolic proteins in normal and neoplastic ovaries by polyacrylamide electrophoresis].

    Voprosy onkologii·2001
    Same author

    [Photoinactivation of bacteriophage PM2 by cyanine dyes].

    Biofizika·2001
    Same author

    [Spontaneous mutants of Alteromonas espejiana, resistant to kanamycin and bleomycin].

    Genetika·2000

    Area of Science:

    • Molecular Biology
    • Virology
    • Microbiology

    Background:

    • Bacteriophage T4 infection involves DNA replication and synthesis of early and late proteins.
    • Viral morphogenesis typically requires coordinated DNA replication and protein synthesis.

    Purpose of the Study:

    • To investigate if bacteriophage T4 morphogenesis can proceed without concurrent phage DNA replication.
    • To analyze the impact of different media conditions on protein synthesis and phage assembly during inhibited DNA replication.

    Main Methods:

    • Infection of E. coli B with T4DtsLB3 under chloramphenicol treatment to block late protein synthesis.
    • Inhibition of phage DNA replication using hydroxyurea and elevated temperature.
    • Culturing in M9 or M9 with amino acids media to assess protein synthesis and assembly.
    • Analysis of capsid protein composition using sucrose density gradients and chloroform lysis.

    Main Results:

    • In M9 medium, primarily early proteins were synthesized.
    • In amino acid-rich medium, DNA synthesis was suppressed and degraded, but both early and late proteins were synthesized.
    • Assembly of phage tails and capsids occurred, with some complete phage particles observed.
    • Isolated capsids lacked specific proteins (Pwac, P23, P22) but contained others (Palt, P20, P23, P24).
    • Viable phage yield ranged from 0.05% to 15% per cell.

    Conclusions:

    • Bacteriophage T4 morphogenesis is achievable without ongoing phage DNA replication.
    • The presence of amino acids supports late protein synthesis and partial phage assembly even when DNA replication is blocked.

    Related Experiment Videos