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Inhibition of stem cell factor- and nerve growth factor-induced morphological change by wortmannin in mast cells
Abstract:
Recombinant murine stem cell factor (rmSCF) or recombinant murine nerve growth factor (rmNGF) induced the morphological change of large numbers of rat peritoneal mast cells (RPMC). We investigated the role of phosphatidylinositol 3'-kinase (PI3-kinase) in receptors-mediated morphological change in RPMC. Exposure of RPMC to PI3-kinase inhibitor, wortmannin, before the addition of rmSCF and rmNGF antagonized those factors-induced morphological change. These results suggest that the Pl3-kinase is involved in the signal transduction pathway responsible for morphological change following stimulation of rmSCF and rmNGF and that wortmannin blocks these responses.
Insights
Recombinant stem cell factor (rmSCF) and nerve growth factor (rmNGF) alter rat mast cell shape. Phosphatidylinositol 3'-kinase (PI3-kinase) inhibition by wortmannin blocked these rmSCF and rmNGF-induced changes, indicating PI3-kinase
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Rat peritoneal mast cells (RPMC) undergo morphological changes when exposed to specific growth factors.
- Recombinant murine stem cell factor (rmSCF) and recombinant murine nerve growth factor (rmNGF) are known to induce these cellular alterations.
- The underlying signaling pathways mediating these morphological changes require further elucidation.
Purpose of the Study:
- To investigate the role of phosphatidylinositol 3 '-kinase (PI3-kinase) in rmSCF and rmNGF-mediated morphological changes in RPMC.
- To determine if PI3-kinase is a critical component of the signal transduction pathway activated by rmSCF and rmNGF.
Main Methods:
- RPMC were isolated from rats.
- Cells were treated with rmSCF or rmNGF.
- The effect of the PI3-kinase inhibitor, wortmannin, on rmSCF and rmNGF-induced morphological changes was assessed.
- Morphological changes were observed and quantified.
Main Results:
- Both rmSCF and rmNGF induced significant morphological changes in a large number of RPMC.
- Pre-treatment of RPMC with wortmannin antagonized the morphological changes induced by both rmSCF and rmNGF.
- Wortmannin effectively blocked the cellular responses to these growth factors.
Conclusions:
- PI3-kinase plays a crucial role in the signal transduction pathway responsible for rmSCF and rmNGF-induced morphological changes in RPMC.
- Wortmannin acts as an effective inhibitor, blocking these specific cellular responses mediated by PI3-kinase.
- These findings highlight the importance of PI3-kinase signaling in mast cell activation and morphology.