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Updated: Jul 1, 2026
14:23
Recombineering Homologous Recombination Constructs in Drosophila
Published on: July 13, 2013
Complementarity-determining region (CDR) implantation: a theme of recombination
E Söderlind1, M Ohlin, R Carlsson
1BioInvent Therapeutic AB, Lund, Sweden. es@bioinvent.com
Summary
Complementarity-determining region (CDR) implantation is a new antibody engineering technology. It creates novel antibody molecules with genetic variability exceeding natural immune system diversity.
Area of Science:
- Antibody engineering
- Immunology
- Molecular biology
Background:
- Traditional antibody engineering methods have limitations in generating diverse antibody libraries.
- The immune system naturally generates antibody diversity through genetic recombination.
Purpose of the Study:
- To introduce and discuss a novel technology called complementarity-determining region (CDR) implantation.
- To highlight the potential of CDR implantation for creating new antibody molecules with enhanced genetic variability.
Main Methods:
- CDR implantation involves the random combination of in vivo-formed CDR sequences.
- These combined CDR sequences are integrated into a single master framework.
- This process allows for the generation of novel gene combinations.
Main Results:
- The technology enables the creation of entirely new antibody gene combinations.
- CDR implantation results in extremely large genetic variability.
- This variability surpasses the natural diversity found in the immune system.
Conclusions:
- CDR implantation represents a significant advancement in antibody engineering.
- The technology offers a powerful tool for generating diverse antibody libraries for selection processes.
- This approach has the potential to accelerate the discovery of novel therapeutic antibodies.
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