Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Prostate specific antigen is detectable in formalin-fixed semen.

R C Eyre1, H Z Yin, A A Kiessling

  • 1Laboratory of Reproductive Biology, Division of Urology, Beth-Israel Deaconess Medical Center, Boston, MA, USA.

BJU International
|May 8, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The right dorsolateral prefrontal cortex is essential in seconds range timing, but not in milliseconds range timing: An investigation with transcranial direct current stimulation.

Brain and cognition·2019
Same author

Inflammation-induced GluA1 trafficking and membrane insertion of Ca<sup>2+</sup> permeable AMPA receptors in dorsal horn neurons is dependent on spinal tumor necrosis factor, PI3 kinase and protein kinase A.

Experimental neurology·2017
Same author

Slow development of ALS-like spinal cord pathology in mutant valosin-containing protein gene knock-in mice.

Cell death & disease·2012
Same author

Invasive pulmonary aspergillosis in patients with HBV-related liver failure.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2011
Same author

Endogenous retrovirus expression in testis and epididymis.

Biochemical Society transactions·2007
Same author

In the stem-cell debate, new concepts need new words.

Nature·2001
Same journal

Cancer detection in the European Randomised Study of Screening for Prostate Cancer (ERSPC).

BJU international·2026
Same journal

Outcomes of maintenance BCG versus gemcitabine/docetaxel following BCG induction in NMIBC.

BJU international·2026
Same journal

A call for regimen agnostic circulating tumour DNA-guided adjuvant selection in muscle-invasive bladder cancer.

BJU international·2026
Same journal

Ablative radiotherapy in castration-resistant prostate cancer.

BJU international·2026
Same journal

Longitudinal circulating tumour DNA identifies patients at high risk of upstaging and recurrence in non-muscle-invasive bladder cancer.

BJU international·2026
Same journal

Venous thromboembolism after penile cancer surgery: a UK PeCaN study.

BJU international·2026
See all related articles

Prostate specific antigen (PSA) and PSA-positive cells are detectable in archived semen. This pilot study found PSA in prostasomes, associated with non-spermatozoal cells, and as amorphous protein, offering insights into prostate physiology.

Area of Science:

  • Andrology
  • Urology
  • Biochemistry

Background:

  • Prostate specific antigen (PSA) is a key biomarker.
  • Detection of PSA in archived semen could offer new diagnostic avenues.
  • Aldehyde fixation is commonly used for semen archiving.

Purpose of the Study:

  • To determine if prostate specific antigen (PSA) and/or PSA-positive cells can be detected and characterized in semen specimens archived in aldehyde fixative.
  • To explore the potential of immunocytological analysis for archived semen.

Main Methods:

  • Pilot study involving 12 men's semen specimens (6 pre-vasectomy, 6 infertility evaluation).
  • Immunocytological assessment using monoclonal antibodies against PSA and CD 45 (control).
  • Analysis of fixed semen elements.

Related Experiment Videos

Main Results:

  • PSA was detectable in semen from fertile men within prostasomes and as amorphous protein.
  • Semen from men undergoing infertility evaluation showed greater variation in PSA forms and amounts.
  • PSA was also found associated with some non-spermatozoal cells (NSCs).

Conclusions:

  • PSA is detectable in aldehyde-fixed semen specimens using immunocytological analysis.
  • Three forms of PSA were identified: within prostasomes, associated with NSCs, and amorphous protein.
  • Variations in detected PSA suggest potential for insights into prostate physiology.