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Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing
Published on: July 10, 2018
Corneal wound healing in tenascin knockout mouse
A Matsuda1, A Yoshiki, Y Tagawa
1Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan.
Investigative Ophthalmology & Visual Science
|May 11, 1999
Summary
Tenascin (TN) is crucial for corneal wound healing, particularly in suture injuries. Its absence impairs stromal cell migration and reduces fibronectin (FN) expression, highlighting TN's significant role in healing under mechanical stress.
Area of Science:
- Extracellular Matrix Biology
- Ophthalmology
- Wound Healing Research
Background:
- Tenascin (TN) is an extracellular matrix glycoprotein found in developing tissues and tumors.
- TN is present in the embryonic cornea and during corneal wound healing, but its specific role is unclear.
Purpose of the Study:
- To investigate the role of Tenascin (TN) in corneal wound healing.
- Utilized Tenascin knockout (TNKO) mice to examine healing processes.
Main Methods:
- Corneal injuries (linear perforation and suture wounds) were created in TNKO and wild-type mice.
- Histological analysis compared wound healing.
- Immunohistochemistry and immunoblot analysis assessed TN and fibronectin (FN) expression.
Main Results:
- No significant difference in healing for linear perforation wounds between groups.
- Suture wounds in TNKO mice showed compressed stroma and reduced keratocyte migration.
- Fibronectin (FN) expression was upregulated but lower in TNKO mice after suture injury compared to wild-type.
Conclusions:
- Tenascin (TN) enhances fibronectin (FN) expression in suture wounds.
- TN deficiency impairs stromal cell migration in the cornea.
- TN plays a vital role in corneal wound healing, especially under mechanical stress.
