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Calcium-binding proteins: differential expression in the rat olfactory cortex after neonatal olfactory bulbectomy
1Department of Psychology, University of Virginia, Charlottesville 22903, USA.
Journal of Neurobiology
|May 11, 1999
Summary
Neonatal olfactory bulb removal alters calcium-binding proteins in the primary olfactory cortex. Calbindin and parvalbumin increased, while calretinin decreased, suggesting neuroprotection.
Area of Science:
- Neuroscience
- Cell Biology
- Neurochemistry
Background:
- EF-hand calcium-binding proteins, including calbindin, parvalbumin, and calretinin, are crucial for intracellular calcium buffering.
- These proteins are found in distinct cell populations within the olfactory bulb and primary olfactory cortex (POC).
Purpose of the Study:
- To quantitatively assess the postnatal expression of calbindin, parvalbumin, and calretinin in layer III of the POC.
- To investigate the effects of neonatal olfactory bulb removal on these calcium-binding proteins.
Main Methods:
- Quantitative immunohistochemistry was employed to examine protein expression.
- The study analyzed protein levels 30 days post-neonatal bulbectomy.
Main Results:
- Calbindin-immunoreactive profiles increased by 68% after bulbectomy.
- Parvalbumin-immunoreactive profiles showed a significant increase of 163%.
- Calretinin-immunoreactive profiles decreased by 46%.
Conclusions:
- Olfactory bulb afferent input regulates the expression of these calcium-binding proteins.
- Increased calbindin and parvalbumin expression may offer neuroprotection against cellular changes induced by neonatal bulb removal.