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Related Experiment Videos

[Reaction between tetranitromethane and deoxyribonucleoproteins].

L A Ptitsyn, M A Chepyzheva, G Ia Kolomiĭtseva

    Biokhimiia (Moscow, Russia)
    |October 1, 1978
    PubMed
    Summary

    This study compared tetranitromethane nitration of deoxyribonucleoprotein (DNP) under UV light and EDTA treatment. Internucleosomic interactions in DNP

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    [Study of DNA-protein crosslinks induced by UV light in Hela cells].

    Biokhimiia (Moscow, Russia)·1994

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Structural Biology

    Context:

    • Deoxyribonucleoprotein (DNP) structure and its modifications are crucial for understanding DNA packaging and function.
    • UV light and chelate agents like EDTA are known to alter DNP structure.
    • Nucleosome structure and higher-order chromatin organization influence DNA accessibility and reactivity.

    Purpose:

    • To comparatively study the kinetics of tetranitromethane nitration of tyrosine residues in DNP.
    • To investigate the effects of UV light and EDTA treatment on DNP structure and nitration patterns.
    • To elucidate the role of internucleosomic interactions in DNP nitration.

    Summary:

    • Tetranitromethane nitration of tyrosine residues in deoxyribonucleoprotein (DNP) was studied kinetically.
    • UV light stabilized DNP structure by forming protein-DNA linkages, while EDTA treatment induced structural transitions.
    • Differences in nitration rates and degrees were attributed to internucleosomic interactions forming the DNP supernucleosomic structure.

    Impact:

    • Provides insights into the structural dynamics of DNP and chromatin organization.
    • Highlights the influence of higher-order chromatin structure on the accessibility of DNA and proteins.
    • Contributes to understanding how structural changes affect molecular interactions within the nucleus.

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