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Preparation of metabolically active cell suspensions from wool roots.

K A Ward

    Australian Journal of Biological Sciences
    |December 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed a method to create metabolically active wool root cell suspensions using trypsin. These cells incorporate labeled molecules and show activity, particularly those from follicle bulbs.

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    Area of Science:

    • Cell Biology
    • Dermatology
    • Biochemistry

    Background:

    • Wool roots contain diverse cell types crucial for hair growth.
    • Understanding wool follicle cell metabolism is key to hair biology research.

    Purpose of the Study:

    • To establish a method for preparing metabolically active cell suspensions from wool roots.
    • To characterize the cell types and metabolic activity within these suspensions.

    Main Methods:

    • Utilized the proteolytic enzyme trypsin for cell dissociation.
    • Optimized trypsin and inorganic ion concentrations.
    • Incorporated EGTA to enhance cell yield.
    • Assessed metabolic activity via radiolabeled precursor incorporation and drug sensitivity.

    Main Results:

    • Successfully prepared heterogeneous cell suspensions resembling follicle bulb cells, keratinocytes, and inner root sheath cells.
    • Determined optimal conditions for trypsin concentration and inorganic ions.
    • EGTA inclusion significantly increased cell yield.
    • Cell suspensions incorporated [14C]leucine, [3H]uridine, and [3H]thymidine, indicating active protein, RNA, and DNA synthesis.
    • Cells demonstrated sensitivity to cycloheximide and emetine, but not chloramphenicol or rifampin, confirming eukaryotic origin.
    • Autoradiography revealed highest metabolic activity in presumed follicle bulb cells.

    Conclusions:

    • A reliable method for generating metabolically active wool root cell suspensions has been established.
    • These suspensions provide a valuable tool for studying wool follicle cell biology and keratinocyte differentiation.
    • Follicle bulb cells exhibit the highest metabolic activity within the prepared suspension.