Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Melatonin in sleep disorders.

Neurologia·2022
Same author

Melatonin in sleep disorders.

Neurologia·2018
Same author

Sterility and Safety Validation for Transport Packaging of Organs and Tissues.

Transplantation proceedings·2018
Same author

Sleep biosignature of Type 2 diabetes: a case-control study.

Diabetic medicine : a journal of the British Diabetic Association·2016
Same author

Synthesis of a heterogeneous artificial metallolipase with chimeric catalytic activity.

Chemical communications (Cambridge, England)·2015
Same author

[Treating acute postoperative pain: evaluation of results on the surgical ward].

Revista espanola de anestesiologia y reanimacion·2011

Related Experiment Video

Updated: Mar 6, 2026

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography
11:01

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography

Published on: January 18, 2020

9.1K

A simple method for quantifying IgM of low molecular weight.

O Romero, C Yantorno, M Romero-Piffiguer

    Journal of Immunological Methods
    |January 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    A novel radial immunodiffusion method accurately quantifies low molecular weight IgM in serum. This simple assay offers a convenient and rapid way to determine IgM concentrations down to 1 mg%.

    More Related Videos

    Quantitative Measurement of Intrathecally Synthesized Proteins in Mice
    08:23

    Quantitative Measurement of Intrathecally Synthesized Proteins in Mice

    Published on: November 29, 2019

    11.9K
    Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
    07:57

    Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

    Published on: August 21, 2019

    9.3K

    Related Experiment Videos

    Last Updated: Mar 6, 2026

    Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography
    11:01

    Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography

    Published on: January 18, 2020

    9.1K
    Quantitative Measurement of Intrathecally Synthesized Proteins in Mice
    08:23

    Quantitative Measurement of Intrathecally Synthesized Proteins in Mice

    Published on: November 29, 2019

    11.9K
    Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
    07:57

    Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

    Published on: August 21, 2019

    9.3K

    Area of Science:

    • Immunology
    • Biochemistry
    • Analytical Chemistry

    Background:

    • Quantitation of low molecular weight IgM is crucial for diagnosing certain immune disorders.
    • Existing methods for IgM quantitation can be complex or time-consuming.

    Purpose of the Study:

    • To introduce a new, simple, and accurate method for quantifying low molecular weight IgM in serum.
    • To establish a convenient and rapid assay for IgM determination.

    Main Methods:

    • Radial immunodiffusion technique.
    • Utilized 7% agar gel for serum sample analysis.
    • Developed a method for precise quantitation of low molecular weight IgM.

    Main Results:

    • The method allows for exact determination of low molecular weight IgM concentrations.
    • Quantitation is possible for very small quantities, down to 1 mg%.
    • Results align with previously established assay procedures.

    Conclusions:

    • The described radial immunodiffusion method is a simple and effective tool for low molecular weight IgM quantitation.
    • This assay provides a convenient and rapid alternative to existing methods.
    • The technique ensures accurate determination of IgM levels in serum samples.