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Provocation of pseudomoniasis with cyclophosphamide in mice
Laboratory Animals
|July 1, 1978
Summary
Cyclophosphamide treatment in mice contaminated with Pseudomonas aeruginosa led to a fatal syndrome. Intestinal P. aeruginosa translocated to target organs, with the liver being the primary site of damage.
Area of Science:
- Immunology
- Microbiology
- Toxicology
Background:
- Cyclophosphamide is an immunosuppressive drug.
- Pseudomonas aeruginosa is an opportunistic pathogen.
- Intestinal translocation of bacteria can lead to systemic infection.
Purpose of the Study:
- To investigate the cause of a fatal syndrome in cyclophosphamide-treated mice contaminated with P. aeruginosa.
- To identify the source and target organs of P. aeruginosa during this syndrome.
Main Methods:
- Mice were treated with cyclophosphamide.
- Mice were experimentally contaminated with P. aeruginosa.
- Clinical signs, mortality, and organ pathology were assessed.
Main Results:
- A fatal syndrome developed in treated mice.
- P. aeruginosa was identified as the causative agent.
- Evidence suggested intestinal translocation of P. aeruginosa.
- The liver was identified as a key target organ.
Conclusions:
- Cyclophosphamide-induced immunosuppression facilitates P. aeruginosa-mediated fatal syndrome.
- Intestinal P. aeruginosa translocation is a critical factor in disease pathogenesis.
- The liver is a primary target organ in this model of P. aeruginosa infection.