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Updated: Aug 17, 2026

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Purification of the M. magneticum Strain AMB-1 Magnetosome Associated Protein MamAΔ41
Published on: March 25, 2010
[Proteins of bacterial membranes. Purification of NADH-dehydrogenase by electrofocusing]
Biokhimiia (Moscow, Russia)
|October 1, 1976
Abstract:
A highly purified preparation of NADH dehydrogenase was isolated from bacteria M. lysodeikticus membranes. The purification procedure involved extraction of the enzyme complex from isolated membranes by EDTA, solubilization of the complex by non-ionogenic detergent (1% Triton X-100), chromatography on DEAE-cellulose and electrofocussing in the pH gradient 4-6. The isoelectric point of the preparation is at 4.5; its main component is a protein with m.w. of about 76.000.

