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Related Experiment Videos

Membrane flow during pinocytosis. A stereologic analysis.

R M Steinman, S E Brodie, Z A Cohn

    The Journal of Cell Biology
    |March 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

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    Horseradish peroxidase (HRP) helps visualize pinocytic vesicles and lysosomes in cells. This study quantifies fluid and membrane uptake rates, revealing rapid vesicle size reduction during lysosome formation.

    Area of Science:

    • Cell Biology
    • Cytochemistry
    • Stereology

    Background:

    • Horseradish peroxidase (HRP) is a cytochemical marker.
    • Pinocytic vesicles and secondary lysosomes are key cellular components.

    Purpose of the Study:

    • To stereologically analyze pinocytic vesicles and secondary lysosomes using HRP.
    • To quantify the rates of fluid and membrane internalization via pinocytosis.
    • To investigate the fate of internalized material and vesicle dynamics.

    Main Methods:

    • Utilized the diaminobenzidine (DAB) technique with HRP as a marker.
    • Employed stereologic analysis to measure vesicle and lysosome dimensions.
    • Calculated fractional volume and surface area flux rates for pinocytosis.

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    Main Results:

    • The DAB technique effectively detects and distinguishes pinocytic vesicles from secondary lysosomes.
    • HRP-filled pinocytic vesicles saturate within 5 minutes; secondary lysosomes saturate in 45-60 minutes.
    • Macrophages and L cells internalize their surface area equivalent every 33 and 125 minutes, respectively.
    • Incoming vesicle volume/surface area is 10x greater than secondary lysosome dimensions per hour, implying rapid size reduction.

    Conclusions:

    • HRP is a reliable marker for studying pinocytosis and lysosome formation.
    • Cells exhibit rapid vesicle size reduction and fluid egress during secondary lysosome formation.
    • Cellular membrane components from vacuoles are likely recycled to the cell surface.