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Updated: Oct 22, 2026
Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
Published on: May 2, 2018
A survey of fungal flora in respiratory therapy equipment
Abstract:
This survey, designed to detect the presence of fungi in respiratory therapy equipment by using a modification of the method of Nazemi et al, indicated significant numbers of fungi in 6 of 30 respiratory therapy devices used in the home, while none of 138 devices used in the hospital showed significant numbers of fungi. Species of fungi detected in large numbers were all yeasts and yeast-like organisms. Additionally, 50% of home devices were found to be moderately to heavily contaminated with either bacteria or fungi, or both, while only 3.6% of the hospital equipment achieved similar levels of contamination (P less than or equal to 0.005), and in these cases, bacteria were the primary contaminants. It was concluded that fungi merited consideration as potential contaminants of respiratory therapy equipment, and that home care respirators presented significantly greater changes for possibly hazardous contamination than did hospital-maintained equipment.
Insights
Home respiratory therapy devices show higher fungal contamination than hospital equipment. This highlights potential risks associated with home-use respirators needing further investigation.
Area of Science:
- Microbiology
- Infectious Diseases
- Biomedical Engineering
Background:
- Respiratory therapy equipment is crucial for patient care.
- Contamination of medical devices can lead to healthcare-associated infections.
- Fungal contamination in respiratory devices requires thorough investigation.
Purpose of the Study:
- To detect and quantify fungal presence in home and hospital respiratory therapy devices.
- To compare contamination levels between home-care and hospital-maintained equipment.
- To identify types of microorganisms contaminating respiratory devices.
Main Methods:
- A modified Nazemi et al. method was employed for fungal detection.
- Samples were collected from 30 home-use and 138 hospital-use respiratory devices.
- Microbial contamination (bacteria and fungi) was assessed quantitatively.
Main Results:
- Significant fungal contamination was found in 6 of 30 (20%) home devices, but none of 138 hospital devices.
- Home devices showed 50% moderate to heavy contamination (bacteria/fungi), versus 3.6% for hospital devices (P <= 0.005).
- Detected fungi were primarily yeasts and yeast-like organisms.
Conclusions:
- Fungi are potential contaminants of respiratory therapy equipment.
- Home-care respirators pose a significantly higher risk of hazardous contamination compared to hospital equipment.
- Regular monitoring and improved disinfection protocols for home respiratory devices are recommended.
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