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Encephalomyocarditis (EMC) virus infection in PC12 and C6 cells
W Su1, A Ueno-Yamanouchi, H Nakayama
1Department of Veterinary Pathology, Faculty of Agriculture, University of Tokyo, Japan. aa77170@hongo.ecc.u-tokyo.ac.jp
Abstract:
PC12 cells derived rom rat pheochromocytoma and C6 cells derived from rat glioma were infected with 0.3 plaque forming units (PFU)/cell of the D variant of encephalomyocarditis virus (EMC-D), after pretreatment with or without nerve growth factor (NGF). The virus titres in medium and cells were investigated at 6, 12, 24, 48 and 72 h post infection (HPI), and histopathology and viral antigens in cells were examined at 24 and 48 HPI, respectively. As a result, neither viral replication nor light and electron microscopic changes were observed in PC12 cell cultures without NGF-pretreatment. On the contrary, in PC12 cell cultures with NGF-pretreatment, the virus titre prominently increased at 12 HPI, and peaked at 48 HPI. In addition, distinct histological and ultrastructural changes with viral antigens in cells were observed. C6 cells showed similar morphology and susceptibility to EMC-D-infection irrespective of NGF-pretreatment. Namely, the virus titres in C6 cell cultures increased slightly and viral antigens were found in a small number of C6 cells, but there were no evident histological and ultrastructural changes. These results suggest that PC12 cells pretreated with NGF and C6 cells are susceptible to EMC-D infection in vitro.
Insights
Nerve growth factor (NGF) pretreatment enhances encephalomyocarditis virus (EMC-D) replication in PC12 cells, leading to observable cellular damage. C6 cells showed limited susceptibility to EMC-D infection regardless of NGF.
Area of Science:
- Virology
- Cell Biology
- Neuroscience
Background:
- Encephalomyocarditis virus (EMC-D) is a known pathogen.
- PC12 cells are derived from rat pheochromocytoma, and C6 cells from rat glioma.
- Nerve growth factor (NGF) plays a role in neuronal development and survival.
Purpose of the Study:
- To investigate the effect of NGF pretreatment on the susceptibility of PC12 and C6 cells to EMC-D infection.
- To analyze viral replication, cellular changes, and viral antigen presence in infected cells.
Main Methods:
- PC12 and C6 cells were infected with EMC-D (0.3 PFU/cell) after pretreatment with or without NGF.
- Virus titers were measured at various time points post-infection (6-72 HPI).
- Histopathology and viral antigens were examined using light and electron microscopy at 24 and 48 HPI.
Main Results:
- PC12 cells without NGF showed no viral replication or cellular changes.
- NGF-pretreated PC12 cells exhibited increased EMC-D titers, peaking at 48 HPI, with significant histological and ultrastructural alterations and viral antigens.
- C6 cells demonstrated slight viral replication and minimal viral antigen presence, irrespective of NGF, with no evident cellular damage.
Conclusions:
- NGF pretreatment significantly enhances EMC-D susceptibility and replication in PC12 cells, inducing cytopathic effects.
- C6 cells are less susceptible to EMC-D infection compared to NGF-treated PC12 cells, showing limited viral replication and no significant pathology.
- These findings highlight the differential impact of NGF on viral infection in neuronal and glial cell models.