Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The mouse major histocompatibility complex: some assembly required.

C Amadou1, A Kumánovics, E P Jones

  • 1Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, USA.

Immunological Reviews
|May 13, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Safety and glycemic outcomes of DBLG1 coupled to the DANA-i pump in adults and adolescents with type 1 diabetes mellitus. The SP8 trial.

Journal of endocrinological investigation·2025
Same author

Effect of Citation Numbers and Team Members on the Possibility of and Time Needed to Complete Screening for Systematic and Scoping Reviews.

Medical reference services quarterly·2025
Same author

Role of immune mediators in predicting hospitalization of SARS-CoV-2 positive patients.

Cytokine·2022
Same author

Comment on Gaudillère et al. Effects of remote care of patients with poorly controlled type 1 diabetes included in an experimental telemonitoring programme [Diabetes Metab 2021 Apr 13; 101251].

Diabetes & metabolism·2021
Same author

Five-year mortality in patients with diabetic foot ulcer during 2009-2010 was lower than expected.

Diabetes & metabolism·2019
Same author

HLA genotyping using the Illumina HLA TruSight next-generation sequencing kits: A comparison.

International journal of immunogenetics·2017
Same journal

Diversity, Equality, and Inclusion in the naïve T Cell Receptor Repertoire.

Immunological reviews·2026
Same journal

Macrophage Plasticity and Immune Remodeling in Ischemic Heart Failure.

Immunological reviews·2026
Same journal

The T Cell Receptor: Molecular Sensor, Therapeutic Mediator and Probabilistic Driver of Adaptive Immunity.

Immunological reviews·2026
Same journal

Tissue-Resident Memory T Cells in the Heart: An Emerging Role in Chronic Inflammation.

Immunological reviews·2026
Same journal

Rethinking Immunity in Tissues: The Biology of Tertiary Lymphoid Structures.

Immunological reviews·2026
Same journal

Inflammation-Driven Lymphoid Structures: Organization, Function, and Clinical Impact Across Autoimmunity, Cancer, and Checkpoint Toxicity.

Immunological reviews·2026
See all related articles

Researchers mapped the mouse major histocompatibility complex (Mhc) using yeast and bacterial artificial chromosome clones. This work details gene organization and conserved synteny with human and rat Mhc regions.

Area of Science:

  • Immunogenetics
  • Comparative Genomics
  • Mammalian Genetics

Background:

  • The mouse major histocompatibility complex (Mhc) is a critical region for immune response and transplantation.
  • Understanding Mhc organization is essential for comparative genomics and disease research.
  • Previous studies have characterized parts of the Mhc, but a complete contig assembly was lacking.

Purpose of the Study:

  • To assemble a comprehensive contig of the mouse Mhc from C57BL/6 and 129 strains using yeast artificial chromosome (YAC) and bacterial artificial chromosome (BAC) clones.
  • To analyze gene content and organization within the Mhc, focusing on class I and class II regions.
  • To investigate conserved synteny between mouse, rat, and human Mhc regions.

Main Methods:

  • Assembly of YAC and BAC clone contigs spanning the mouse Mhc.

Related Experiment Videos

  • Detailed analysis of the proximal 1 Mb of the H2-M region.
  • Comparative analysis of gene order and content with human and rat Mhc.

    • Main Results:

    • An 8 Mb YAC contig encompassing the entire Mhc from C57BL/6 (H2b) was assembled.
    • A BAC contig for strain 129 (H2bc) was initiated, covering Tapasin to D17Leh89 with identified gaps.
    • The proximal H2-M region analysis revealed 21 class I genes/fragments, 14 olfactory receptor-like genes, and conserved synteny with human and rat Mhc.

    Conclusions:

    • The assembled contigs provide a framework for detailed Mhc analysis and sequencing.
    • The study identified conserved gene organization across species, highlighting the evolutionary importance of the Mhc.
    • Further research on the identified gaps and gene families will enhance our understanding of Mhc function and evolution.