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Effect of lansoprazole on human leukocyte function.
E Capodicasa1, F De Bellis, M A Pelli
1Institute of Internal Medicine and Oncological Sciences, Perugia University, Italy.
Immunopharmacology and Immunotoxicology
|May 13, 1999
Summary
Proton pump inhibitors like lansoprazole can suppress immune cell functions. This study shows lansoprazole inhibits natural killer cell activity and polymorphonucleated cell functions in vitro.
Area of Science:
- Immunology
- Pharmacology
Background:
- Proton pump inhibitors (PPIs) are widely used for acid-related disorders.
- Recent research suggests omeprazole may affect leukocyte functions.
- This raises questions about PPIs' potential immunomodulatory effects.
Purpose of the Study:
- To investigate the in vitro effects of lansoprazole on human leukocyte functions.
- Specifically, to assess its impact on natural killer (NK) cell cytotoxicity, chemotaxis, and polymorphonucleated cell (PMN) superoxide anion generation.
Main Methods:
- NK cell cytotoxicity was measured using a 51Cr release assay.
- PMN chemotaxis was evaluated via an under agarose method.
- Superoxide anion (O2*-) generation was analyzed by reduced cytochrome C.
Main Results:
- Lansoprazole significantly inhibited NK cell activity in a dose-dependent manner.
- Lansoprazole also demonstrated dose-dependent inhibition of PMN functions.
- These effects were observed at concentrations ranging from 100 to 1,000 microM.
Conclusions:
- Lansoprazole, similar to omeprazole, inhibits key leukocyte functions in vitro.
- This suggests that proton pump inhibitors can modulate immune cell activity.
- The immunomodulatory effects are dose-dependent and observed at specific concentrations.