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Orthophosphate is a non-essential activator of Vigna radiata flavokinase
K Das-Panja1, V S Jonnalagadda, S Jonnalagadda
1Department of Biochemistry, University College of Science, Osmania University, Hyderabad, India.
Abstract:
The ATP-dependent phosphorylation of riboflavin to FMN by flavokinase from Vigna radiata was activated by orthophosphate (Pi) in a concentration dependent manner. Pi affected both the K(m) and Vmax, indicating that it is a non-essential, mixed type activator. The extent of activation by Pi was dependent on the cation (Mg2+ or Zn2+). Activation by other anions could be correlated to similarity to Pi in molecular size and structure. These observations suggest the presence of a binding site(s) for a phosphate-like anion on flavokinase.
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