Related Experiment Videos
c-MYC interacts with INI1/hSNF5 and requires the SWI/SNF complex for transactivation function
S W Cheng1, K P Davies, E Yung
1Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Nature Genetics
|May 13, 1999
Summary
The SWI/SNF complex is essential for c-MYC-mediated gene activation. The interaction between c-MYC and INI1 facilitates the recruitment of this complex, impacting gene transcription regulation.
Area of Science:
- Molecular Biology
- Gene Regulation
- Chromatin Remodeling
Background:
- Chromatin organization is crucial for regulating gene expression.
- The SWI/SNF complex remodels chromatin for ATP-dependent transcription activation.
- Components like BRG1, hBRM, and INI1/hSNF5 are involved in cell-cycle arrest and tumor suppression.
Purpose of the Study:
- To investigate the interaction between INI1 and c-MYC.
- To elucidate the role of the SWI/SNF complex in c-MYC-mediated transactivation.
Main Methods:
- Yeast two-hybrid system to identify INI1-interacting proteins.
- In vitro and in vivo interaction studies.
- Analysis of protein domain requirements for interaction.
- Dominant-negative inhibition assays using deletion mutants and ATPase mutants.
Main Results:
- c-MYC was identified as an INI1-interacting protein.
- The interaction between c-MYC and INI1 was confirmed both in vitro and in vivo.
- Specific domains (c-MYC bHLH-Zip, INI1 Rpt1) were found essential for the interaction.
- INI1 deletion fragments and BRG1/hSNF2 ATPase mutants inhibited c-MYC transactivation.
Conclusions:
- The SWI/SNF complex is required for c-MYC-mediated transactivation.
- The c-MYC-INI1 interaction plays a role in recruiting the SWI/SNF complex to target genes.
- This interaction is a key mechanism in regulating gene expression via chromatin remodeling.