Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Src promotes PKCdelta degradation.

R A Blake1, P Garcia-Paramio, P J Parker

  • 1SUGEN Inc., South San Francisco, California 94080-4811, USA.

Cell Growth & Differentiation : the Molecular Biology Journal of the American Association for Cancer Research
|May 13, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Abdominal Aortic Junctional Tourniquet - Stabilized (AAJTS) can be applied both successfully and rapidly by Combat Medical Technicians (CMTs).

BMJ military health·2021
Same author

Non-battle injury among repatriated UK armed forces since cessation of combat operations: a prospective observational study.

Journal of the Royal Army Medical Corps·2017
Same author

Targeting protein kinase C in sarcoma.

Biochimica et biophysica acta·2014
Same author

Prevention of perinatal hepatitis B virus transmission in an obstetric/infant population.

The Canadian journal of infectious diseases = Journal canadien des maladies infectieuses·2012
Same author

Changes in surgical workload at the JF Med Gp Role 3 Hospital, Camp Bastion, Afghanistan, November 2008-November 2010.

Injury·2012
Same author

Initial extremity war wound debridement: a multidisciplinary consensus.

Journal of the Royal Army Medical Corps·2011
Same journal

Early changes in intramitochondrial cardiolipin distribution during apoptosis.

Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research·2002
Same journal

Increased K-ras protein and activity in mouse and human lung epithelial cells at confluence.

Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research·2002
Same journal

Heme deficiency interferes with the Ras-mitogen-activated protein kinase signaling pathway and expression of a subset of neuronal genes.

Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research·2002
Same journal

Early cycling-independent changes to p27, cyclin D2, and cyclin D3 in differentiating mouse embryonal carcinoma cells.

Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research·2002
Same journal

Translational regulation of cyclin D1 by 15-deoxy-delta(12,14)-prostaglandin J(2).

Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research·2002
Same journal

Malignant transformation in human chondrosarcoma cells supported by telomerase activation and tumor suppressor inactivation.

Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research·2002
See all related articles

Platelet-derived growth factor BB (PDGF) stimulates DNA synthesis via Src kinases. Research shows Protein Kinase C delta (PKCδ) is phosphorylated and degraded by Src, inhibiting DNA synthesis, suggesting PKCδ negatively regulates cell cycle progression.

Area of Science:

  • Cellular signaling
  • Molecular biology
  • Cancer research

Background:

  • Platelet-derived growth factor BB (PDGF) is crucial for cell proliferation.
  • Src family tyrosine kinases mediate PDGF signaling, but downstream pathways are unclear.
  • Protein Kinase C (PKC) isoforms are involved in various cellular processes, including cell cycle regulation.

Purpose of the Study:

  • Investigate the signaling interplay between Src and PKC isoforms.
  • Determine the role of PKC isoforms in PDGF-stimulated DNA synthesis.
  • Elucidate the mechanism by which Src influences DNA synthesis.

Main Methods:

  • Studied Src and PKC isoform interactions using cell-based assays.
  • Analyzed protein phosphorylation and degradation.

Related Experiment Videos

  • Utilized mutagenesis to identify critical phosphorylation sites.
  • Assessed the impact of PKC isoform expression on DNA synthesis.

    • Main Results:

    • Src promotes tyrosine phosphorylation and degradation of PKCδ.
    • Enforced expression of PKCδ inhibits PDGF-stimulated DNA synthesis.
    • PKCα and PKCε expression did not affect DNA synthesis.
    • Tyrosine 311 on PKCδ was identified as a critical Src phosphorylation site, mediating degradation.

    Conclusions:

    • PKCδ is a downstream target of Src in PDGF signaling.
    • PKCδ negatively regulates G1-to-S-phase progression.
    • Src-induced degradation of PKCδ is dependent on its tyrosine phosphorylation.
    • PKCδ does not play a positive role in Src-mediated promotion of DNA synthesis.