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Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes
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Extracellular protons activate K+ current in a subpopulation of frog taste receptor cells.

Y V Bobkov1, S S Kolesnikov

  • 1Institute of Cell Biophysics, Russian Academy of Science, Pushchino, Moscow. bobkov@venus.iteb.serpukhov.su

Neuroscience Letters
|May 13, 1999
PubMed
Summary
This summary is machine-generated.

Two frog taste receptor cell types respond differently to acid. Group B cells show increased potassium (K+) conductance when pH lowers, suggesting they possess H+ gated K+ channels.

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Whole-Mount Staining, Visualization, and Analysis of Fungiform, Circumvallate, and Palate Taste Buds
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Published on: February 11, 2021

Area of Science:

  • Neuroscience
  • Sensory Biology
  • Cell Physiology

Background:

  • Taste receptor cells (TRCs) mediate the sense of taste.
  • Different TRC subpopulations exist, each potentially responding to specific stimuli.
  • Acidic stimuli are known to elicit taste responses, but the underlying mechanisms are not fully elucidated.

Purpose of the Study:

  • To identify and characterize distinct taste receptor cell subpopulations in frog taste disks.
  • To investigate the electrophysiological responses of these subpopulations to acid stimuli.
  • To explore the ion channel mechanisms responsible for pH-dependent responses in TRCs.

Main Methods:

  • Morphological and electrophysiological criteria were used to classify TRCs.
  • Patch clamp technique was employed to study ion channel activity.
  • Responses to varying bath solution pH were recorded.

Main Results:

  • Two distinct TRC subpopulations, group A and group B, were identified.
  • Group A TRCs were depolarized by acid stimuli.
  • Group B TRCs exhibited hyperpolarizing responses to acid, with increased K+ conductance as pH decreased.

Conclusions:

  • Frog taste disks contain at least two functionally distinct TRC subpopulations.
  • Group B TRCs likely mediate acid taste perception through a mechanism involving H+ gated K+ channels.
  • These findings contribute to understanding the cellular basis of taste transduction.