Related Experiment Videos
[Gentamicin resistance of enterococcus genus strains isolated from clinical material]
D Wojciechowska1, G Janicka, K Hareńska
1Zakład Diagnostyki Mikrobiologicznej, Samodzielnego Publicznego Zakładu Opieki Zdrowotnej w Zninie.
Abstract:
The occurrence of High Level Gentamicin Resistance (HLGR) was tested among the strains of Enterococcus genus isolated from material potent to microbiological laboratory. The investigation included 184 strains of enterococci, and among them 178 strains of Enterococcus faecalis (96.7%) and 6 strains of Enterococcus faecium (3.3%). HLGR was detected with disks-diffusion method with disks containing 120 micrograms of gentamicin. The screen method with MHA-2 containing 500 mg/l of gentamicin in doubtful results was used. The resistance to high concentration of gentamicin was detected in 29 strains of enterococci (15.76%). The highest percentage of HLGR was proved among strains of enterococci isolated from pyogenic material of hospitalised patients (27.3%).
Insights
High Level Gentamicin Resistance (HLGR) was found in 15.76% of Enterococcus strains. Resistance was highest in strains from hospital-acquired infections, indicating a significant clinical concern.
Area of Science:
- Microbiology
- Antimicrobial Resistance
- Clinical Infectious Diseases
Context:
- Enterococcus species are significant human pathogens.
- High Level Gentamicin Resistance (HLGR) is a critical factor in treatment decisions for enterococcal infections.
- The prevalence of HLGR in clinical isolates requires ongoing surveillance.
Purpose:
- To determine the occurrence of High Level Gentamicin Resistance (HLGR) among Enterococcus strains.
- To identify the prevalence of HLGR in different Enterococcus species.
- To assess the association between HLGR and the source of clinical material.
Summary:
- A total of 184 Enterococcus strains were analyzed, predominantly Enterococcus faecalis (96.7%).
- High Level Gentamicin Resistance (HLGR) was detected in 29 strains (15.76%) using disk diffusion and MHA-2 screening methods.
- The highest prevalence of HLGR (27.3%) was observed in enterococcal strains isolated from pyogenic material of hospitalized patients.
Impact:
- This study highlights a notable prevalence of HLGR in clinical Enterococcus isolates.
- Findings underscore the importance of routine HLGR screening in clinical microbiology laboratories.
- Understanding HLGR prevalence is crucial for guiding effective antibiotic therapy and infection control strategies.