Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA methylation and chromatin modification.

H H Ng1, A Bird

  • 1Institute of Cell and Molecular Biology, University of Edinburgh, King's Buildings, Edinburgh, EH9 3JR, UK. Huck.Hui.Ng@ed.ac.uk

Current Opinion in Genetics & Development
|May 14, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Provenance of late Pleistocene loess in central and eastern Europe: isotopic evidence for dominant local sediment sources.

Scientific reports·2025
Same author

Using morphometrics to identify sex in the eastern blue-tongued skink (Tiliqua scincoides).

Australian veterinary journal·2023
Same author

Blood product utilisation for coronary artery bypass graft surgery at a public and a private hospital in Western Cape Province, South Africa.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2021
Same author

Correction: Comparison of the Non-VKA Oral Anticoagulants Apixaban, Dabigatran, and Rivaroxaban in the Extended Treatment and Prevention of Venous Thromboembolism: Systematic Review and Network Meta-Analysis.

PloS one·2016
Same author

Vascular actions of relaxin: nitric oxide and beyond.

British journal of pharmacology·2016
Same author

Comparison of the Non-VKA Oral Anticoagulants Apixaban, Dabigatran, and Rivaroxaban in the Extended Treatment and Prevention of Venous Thromboembolism: Systematic Review and Network Meta-Analysis.

PloS one·2016
Same journal

Temporal trajectories underlying adult neuronal diversity.

Current opinion in genetics & development·2026
Same journal

Transcription regulation of cell fate plasticity - from embryonic development to tissue regeneration.

Current opinion in genetics & development·2026
Same journal

Shared molecular and cellular programs during regeneration of glandular epithelia.

Current opinion in genetics & development·2026
Same journal

Lineage tracing in human cortical development.

Current opinion in genetics & development·2026
Same journal

Cis-regulatory strategies in developmental patterning.

Current opinion in genetics & development·2026
Same journal

GABAergic neuron fate specification and lineage allocation: from development to disorder.

Current opinion in genetics & development·2026
See all related articles

DNA methylation patterns chromatin structure through methyl-CpG binding protein 2 (MeCP2) interaction with histone deacetylase complexes. Inhibiting these enzymes can reverse DNA methylation-induced gene silencing, linking methylation to chromatin modification.

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Gene Regulation

Background:

  • DNA methylation and chromatin modification are key regulators of gene expression.
  • Methyl-CpG binding protein 2 (MeCP2) plays a role in transcriptional silencing.
  • A link between DNA methylation and chromatin structure has long been observed.

Purpose of the Study:

  • To elucidate the mechanism by which MeCP2 mediates transcriptional silencing.
  • To explain the relationship between DNA methylation and chromatin structure.
  • To investigate the role of histone deacetylases in DNA methylation-mediated gene silencing.

Main Methods:

  • Investigating the interaction between MeCP2 and the Sin3/histone deacetylase co-repressor complex.
  • Utilizing specific histone deacetylase inhibitors.

Related Experiment Videos

  • Assessing the reactivation of silenced genes and reporter constructs.

    • Main Results:

    • MeCP2 interacts with the Sin3/histone deacetylase co-repressor complex.
    • Inhibition of histone deacetylases can reactivate genes silenced by DNA methylation.
    • This interaction provides a mechanistic link between DNA methylation and chromatin structure.

    Conclusions:

    • DNA methylation can pattern chromatin modification through the MeCP2-histone deacetylase pathway.
    • Histone deacetylase activity is crucial for maintaining DNA methylation-induced gene silencing.
    • These findings offer a mechanistic explanation for the interplay between DNA methylation and chromatin structure.