Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nuclear compartments and gene regulation.

M Cockell1, S M Gasser

  • 1Swiss Institute for Experimental Cancer Research, Ch. des Boveresses 155, CH-1066, Epalinges s/Lausanne, Switzerland,

Current Opinion in Genetics & Development
|May 14, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nutrition and the biology of human ageing: molecular mechanisms underlying ageing.

The journal of nutrition, health & aging·2013
Same author

The composition and morphology of yeast nuclear scaffolds.

Journal of cell science·2011
Same author

Repetitive transgenes in C. elegans accumulate heterochromatic marks and are sequestered at the nuclear envelope in a copy-number- and lamin-dependent manner.

Cold Spring Harbor symposia on quantitative biology·2011
Same author

The function of telomere clustering in yeast: the circe effect.

Cold Spring Harbor symposia on quantitative biology·2005
Same author

Long-range silencing and position effects at telomeres and centromeres: parallels and differences.

Cellular and molecular life sciences : CMLS·2003
Same author

Chromosome dynamics in the yeast interphase nucleus.

Science (New York, N.Y.)·2001

Advanced fluorescence microscopy reveals nuclear compartments. Gene silencing correlates with proximity to heterochromatin or the nuclear periphery, impacting gene expression.

Area of Science:

  • Cell Biology
  • Genetics
  • Microscopy

Background:

  • Recent advancements in fluorescence microscopy enable detailed exploration of nuclear organization.
  • The nucleus contains distinct subdomains or compartments characterized by specific factor enrichments.
  • Previous studies suggest a link between gene silencing and proximity to heterochromatic regions or the nuclear periphery.

Purpose of the Study:

  • To investigate the three-dimensional organization of the nucleus using cutting-edge microscopy.
  • To examine the formation, persistence, and influence of nuclear compartments on gene expression.
  • To correlate gene silencing with the spatial positioning within the nucleus.

Main Methods:

  • Utilizing high-resolution fluorescence microscopy techniques.

Related Experiment Videos

  • Applying genetic approaches to study nuclear organization.
  • Analyzing the spatial relationships between genes, heterochromatin, and the nuclear periphery.

    • Main Results:

    • Identification of specific nuclear subdomains based on factor enrichment.
    • Demonstration of correlations between gene silencing and proximity to heterochromatic compartments.
    • Evidence linking gene location relative to nuclear structures with gene expression levels.

    Conclusions:

    • Nuclear compartmentalization plays a significant role in regulating gene expression.
    • The spatial organization within the nucleus, particularly proximity to heterochromatin, is crucial for gene silencing.
    • High-resolution microscopy and genetics are powerful tools for dissecting nuclear architecture and function.