Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Euchromatin01:01

Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chromatin structure mapping in Saccharomyces cerevisiae in vivo with DNase I.

Nucleic acids research·2001
Same author

Tup1p represses Mcm1p transcriptional activation and chromatin remodeling of an a-cell-specific gene.

The EMBO journal·2000
Same author

DNA methyltransferases as probes for chromatin structure in yeast.

Methods in molecular biology (Clifton, N.J.)·2000
Same author

The organized chromatin domain of the repressed yeast a cell-specific gene STE6 contains two molecules of the corepressor Tup1p per nucleosome.

The EMBO journal·2000
Same author

High-resolution structural analysis of chromatin at specific loci: Saccharomyces cerevisiae silent mating-type locus HMRa.

Molecular and cellular biology·1999
Same author

DNA methyltransferases as probes of chromatin structure in vivo.

Methods in enzymology·1999

Related Experiment Video

Updated: Jul 22, 2026

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
07:41

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Published on: October 2, 2017

In vivo methods to analyze chromatin structure.

R T Simpson1

  • 1Department of Biochemistry and Molecular Biology, The Pennsylvania State University, 308 Althouse, University Park, Pennsylvania 16802, USA. rts4@psu.edu

Current Opinion in Genetics & Development
|May 14, 1999
PubMed
Summary

Recent advances enable in vivo chromatin structure assessment, overcoming traditional technical hurdles. Studying intact cells offers a more accurate understanding of DNA regulation in transcription and repair.

More Related Videos

Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis
09:26

Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis

Published on: March 23, 2021

A Multilabel Single Molecule Localization Microscopy Protocol for Investigation of Chromatin in the Dense Nuclear Environment
08:49

A Multilabel Single Molecule Localization Microscopy Protocol for Investigation of Chromatin in the Dense Nuclear Environment

Published on: June 5, 2026

Related Experiment Videos

Last Updated: Jul 22, 2026

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
07:41

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Published on: October 2, 2017

Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis
09:26

Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis

Published on: March 23, 2021

A Multilabel Single Molecule Localization Microscopy Protocol for Investigation of Chromatin in the Dense Nuclear Environment
08:49

A Multilabel Single Molecule Localization Microscopy Protocol for Investigation of Chromatin in the Dense Nuclear Environment

Published on: June 5, 2026

Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • Growing interest in chromatin structure's role in DNA functions like transcription, replication, recombination, and repair.
  • Genetic studies revealed histone mutations impact transcription, and specific genes alter chromatin structure.
  • Recent focus on transcription factors and histone-modifying enzymes influencing chromatin structure.

Purpose of the Study:

  • To address the limited experimental reports on chromatin structure.
  • To overcome technical difficulties associated with traditional structure inference methods.
  • To introduce and validate new methods for assessing chromatin structure in vivo.

Main Methods:

  • Development of novel methods for in vivo chromatin structure assessment.
  • Utilizing intact cells for structural analysis to prevent component loss or rearrangement.
  • Simplifying analysis by avoiding organelle isolation.

Main Results:

  • New methods allow for the assessment of various aspects of chromatin structure within intact cells.
  • In vivo studies mitigate issues related to sample preparation and component stability.
  • The approach simplifies the analysis of chromatin structure and its functional implications.

Conclusions:

  • In vivo methods provide a more reliable approach to studying chromatin structure.
  • These advancements facilitate a deeper understanding of DNA regulation.
  • Future research can leverage these techniques to explore chromatin's role in cellular processes.