Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Anti-sigma factors.

J D Helmann1

  • 1Section of Microbiology, Wing Hall, Cornell University, Ithaca, NY 14853-8101, USA. jdh9@cornell.edu

Current Opinion in Microbiology
|May 14, 1999
PubMed
Summary

Anti-sigma factors regulate gene expression by interacting with alternative sigma factors. Recent research reveals their diverse regulatory mechanisms and structural insights, including holoenzyme destabilization and partner-switching modules.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Global transcriptional response of Bacillus subtilis to heat shock.

Journal of bacteriology·2001
Same author

Roles of metal ions and hydrogen peroxide in modulating the interaction of the Bacillus subtilis PerR peroxide regulon repressor with operator DNA.

Molecular microbiology·2001
Same author

OhrR is a repressor of ohrA, a key organic hydroperoxide resistance determinant in Bacillus subtilis.

Journal of bacteriology·2001
Same author

FosB, a cysteine-dependent fosfomycin resistance protein under the control of sigma(W), an extracytoplasmic-function sigma factor in Bacillus subtilis.

Journal of bacteriology·2001
Same author

The -10 region is a key promoter specificity determinant for the Bacillus subtilis extracytoplasmic-function sigma factors sigma(X) and sigma(W).

Journal of bacteriology·2001
Same author

Mutations in multidrug efflux homologs, sugar isomerases, and antimicrobial biosynthesis genes differentially elevate activity of the sigma(X) and sigma(W) factors in Bacillus subtilis.

Journal of bacteriology·2000

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Anti-sigma factors are key regulators of gene expression controlled by alternative sigma factors.
  • Their activity is modulated through various mechanisms including secretion, sequestration, and interaction with external effectors.

Purpose of the Study:

  • To summarize recent advances in understanding anti-sigma factor regulation.
  • To highlight novel findings regarding their structure, function, and interaction mechanisms.

Main Methods:

  • Genetic analysis of sigma/anti-sigma binding surfaces.
  • Biochemical studies on FlgM holoenzyme destabilization and folding.
  • Structural determination of anti-sigma antagonists.
  • Dissection of partner-switching modules in Bacillus subtilis.

Main Results:

  • Elucidation of specific binding surfaces between sigma and anti-sigma factors.
  • Demonstration of FlgM's role in destabilizing holoenzymes and coupled folding.
  • First structural data for an anti-sigma antagonist.
  • Detailed characterization of two complex partner-switching systems.

Conclusions:

  • Anti-sigma factors employ diverse strategies for regulation, impacting gene expression networks.
  • Recent structural and mechanistic studies provide deep insights into their function.
  • Further research on these factors can uncover new regulatory pathways and potential targets.

Related Experiment Videos