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A comparison of gel-based, nylon filter and microarray techniques to detect differential RNA expression in plants
1Disease Resistance Group, Trait and Technology Development, Pioneer Hi-Bred International Inc., 7300 NW 62nd Ave., Johnston, IA 50131-1004,USA. baldwinda@phibred.com
Current Opinion in Plant Biology
|May 14, 1999
Summary
Plant genomics enables large-scale gene expression monitoring. Advanced RNA assays offer high accuracy for profiling plant gene expression, advancing plant molecular biology research.
Area of Science:
- Plant Science
- Genomics
- Molecular Biology
Background:
- Plant genomics facilitates unprecedented monitoring of gene expression.
- Advancements in multiplexed RNA assays are crucial for understanding plant molecular biology.
- Current techniques provide high-accuracy expression profiles for many plant genes.
Purpose of the Study:
- To review techniques contributing to RNA transcript analysis in plant genomics.
- To highlight the current capabilities and limitations of gene expression profiling methods.
Main Methods:
- Review of three key techniques for RNA transcript analysis.
- Focus on multiplexed assays for RNA abundance measurement.
Main Results:
- Each reviewed technique can profile expression levels for a large, though incomplete, set of plant genes.
- High levels of accuracy are reproducibly achievable with these methods.
- Methods offer a range of labor and financial expenses.
Conclusions:
- Multiplexed RNA assays are rapidly developing and essential for plant molecular biology.
- These techniques provide valuable insights into plant gene expression patterns.
- Future combinations of assays will offer comprehensive views of plant molecular systems.