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Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
12:03

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Published on: July 8, 2012

Self-renewal, differentiation or death: regulation and manipulation of hematopoietic stem cell fate

J Domen1, I L Weissman

  • 1Dept of Pathology and Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305-5428, USA. domen@stanford.edu

Molecular Medicine Today
|May 14, 1999
PubMed

Hematopoietic stem cells (HSCs) are the rare cells from which all hematopoietic cells are derived. The absence of HSCs is not compatible with life because many essential cells, such as myeloid and erythroid cells, are short lived. The hematopoietic system is the first essential organ system that fails following cytotoxic treatments. It is the vulnerability of HSCs that prevents regeneration following treatment and thus long-term survival. Because HSCs have the capacity to regenerate a functional hematopoietic system, the manipulation of these cells in vitro holds many promises for gene-therapeutic and other applications; however, these are severely curtailed by current difficulties in maintaining and expanding HSCs in culture. This review focuses on recent approaches towards understanding how the HSC compartment is regulated in vivo and discusses how this knowledge might be applied to manipulating HSC numbers.

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