Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genetic variation in trophectoderm function in parthenogenetic mouse embryos.

K E Latham1, K Kutyna, Q Wang

  • 1Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA. keith@cleo.fels.temple.edu

Developmental Genetics
|May 14, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular control of mitochondrial function in developing rhesus monkey oocytes and preimplantation-stage embryos.

Reproduction, fertility, and development·2008
Same author

Systems biology of the 2-cell mouse embryo.

Cytogenetic and genome research·2004
Same author

Maternal and environmental factors in early cloned embryo development.

Cytogenetic and genome research·2004
Same author

Reproductive semi-cloning respecting biparental origin. A biologically unsound principle.

Human reproduction (Oxford, England)·2003
Same author

Genetic and epigenetic factors affecting blastomere fragmentation in two-cell stage mouse embryos.

Biology of reproduction·2001
Same author

Role of protein synthesis in the development of a transcriptionally permissive state in one-cell stage mouse embryos.

Biology of reproduction·2001

Maternal genotype impacts early embryo development, affecting trophectoderm function and hatching ability in parthenogenetic embryos. Differences between parthenogenetic and fertilized embryos suggest imprinted genes influence blastocyst development.

Area of Science:

  • Developmental Biology
  • Genetics
  • Reproductive Science

Background:

  • The oocyte provides essential factors for early embryonic development and epigenetic programming.
  • Parthenogenetic embryos are valuable models for studying maternal contributions and genomic imprinting.
  • Maternal genotype influences embryonic development, particularly trophectoderm function.

Purpose of the Study:

  • To investigate the impact of maternal genotype on trophectoderm function in parthenogenetic embryos.
  • To compare the development of parthenogenetic embryos with fertilized embryos.
  • To explore the role of maternal factors and imprinted genes in early embryonic development.

Main Methods:

  • Generation and culture of parthenogenetic embryos from (B6D2)F1 and C57BL/6 oocytes.

Related Experiment Videos

  • Assessment of hatching efficiency and blastocoel re-expansion after cytoskeletal inhibitor treatment.
  • Quantification of Na+, K(+)-ATPase alpha 1 subunit mRNA abundance.

    • Main Results:

    • (B6D2)F1 parthenogenones exhibit more efficient hatching than C57BL/6 parthenogenones.
    • Parthenogenones show faster blastocoel re-expansion than fertilized embryos.
    • Maternal genotype affects trophectoderm function, with (B6D2)F1 parthenogenones displaying higher Na+, K(+)-ATPase alpha 1 subunit mRNA levels.

    Conclusions:

    • Maternal genotype significantly influences trophectoderm function in the absence of paternal contribution.
    • Differences between parthenogenetic and fertilized embryos suggest a role for imprinted genes in hatching and blastocoel expansion.
    • Variations in hatching and re-expansion may stem from differences in hatching enzymes or factors regulating fluid balance and Na+, K(+)-ATPase activity.