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[Damage effects of asbestos and cigarette smoke solution on human embryo lung cell DNA]
Objective:
To study the combined damage effects of asbestos and cigarette solution on human embryo lung cell (HEL) DNA.
Methods:
Unscheduled DNA synthesis (UDS) assay was used. Repair and synthesis on DNA in HEL treated with asbestos and/or cigarette smoke was studied.
Results:
It showed that UDS in HEL could be induced by exposure to asbestos or cigarette smoke only with a significant dose-response relationship, and the amounts of [3H]-TdR incorporation in cells treated with combination of asbestos and cigarette smoke was significantly higher than their sum in cells treated with asbestos or cigarette only.
Conclusion:
There is a synergistic damage effect of asbestos and cigarette on DNA of HEL. In addition, dimethyl sulfoxide (DMSO), a scavenger of .OH, can partly inhibit [3H]-TdR incorporation caused by asbestos. And .OH plays certain role in damage to DNA of cells caused by asbestos.