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[Adaptive response of CHL to hydrogen peroxide induced oxidative stress and the mechanism]
Objective:
To study peroxidative adaptive response of eukaryocyte and its mechanisms.
Methods:
Chinese hamster fibroblast cells (CHL) pretreated with low concentration of hydrogenperoxide (H2O2) were retreated by the following high concentration of H2O2. Cellular viability (MTT method) and DNA strand break (FADU method), as adaptive-acting indicators were selected. Meanwhile, CAT activity and PARP were separately determined on the adaptive response to DNA strand break.
Results:
There were peroxidative adaptive responses to non-lethal damage of high concentration H2O2 in both cellular viability and DNA strand break levels; but adaptive models (conditions) between the two levels were different. The CAT activity induced by the low level of H2O2 was enhanced and related to peroxidative adaptation to DNA break. Furthermore, PARP blocker, 3-AB, markedly inhibited the adaptive response to DNA-strand break.
Conclusion:
Two models adaptation may be via different inducible adaptive pathways. CAT and PARP participated in the induction of peroxidative adaptation to DNA strand break.