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[Preparation of monoclonal antibodies against hepatitis E and its application]
Abstract
Objective:
To study the particle of hepatitis E virus (HEV) and its significance in specific diagnosis for hepatitis E (HE).
Methods:
Four strains of monoclonal antibodies (McAb) against HEV, 5B12, 5F1, 5B9 and 4G10, were prepared with HEV antigen (used as immunogen) expressed by genetic engineering and detected by indirect enzyme-linked immunosorbent assay (ELISA) with purified synthetic polypeptide antigen.
Results:
The monoclonal anti-HEV prefared could produce inhibitory reaction with anti-HEV positive serum and combined specifically with HEV particle forming virus-antibody complex seen clearly under electron microscope.
Conclusion:
McAb against HEV so prepared with high specificity can be used to detect and identify HEV.