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Inhibitory effects of vinpocetine on sodium current in rat cardiomyocytes
1Department of Biophysics, School of Basic Medical Sciences, Shanghai Medical University, China.
Aim:
To study the effects of vinpocetine (Vin) on the sodium current (INa) in cardiomyocytes.
Methods:
The sodium current in adult rat ventricular myocytes was measured by whole cell patch-clamp technique.
Results:
The INa in cardiomyocytes was blocked reversibly by Vin, in concentration-dependent and voltage-dependent manner, but not rate- or use-dependent. The INa was attenuated by 13%-75% when the Vin concentration was raised from 10 to 80 mumol.L-1. The IC50 (95% confidence limits) was 36.4 (28.1-47.1) mumol.L-1. When the membrane potential depolarized over the range of -90 mV to +40 mV in 10-mV step, inhibitory effect of Vin on the INa was 39% at first, then maintained at a higher level, about 52% +/- 5%. The maximal depression (57%) reached at about 0 mV. Vin influenced both the activation and inactivation processes of sodium channel, and resulted in attenuation of the window currents (the slowly inactivating sodium currents).
Conclusion:
Vin inhibited sodium currents in rat ventricular myocytes.
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