High mobility group (HMG) non-histone chromosomal proteins HMG1 and HMG2 are significant target antigens of perinuclear anti-neutrophil cytoplasmic antibodies in autoimmune hepatitis
Summary
This summary is machine-generated.High mobility group (HMG) proteins HMG1 and HMG2 are identified as key targets for perinuclear anti-neutrophil cytoplasmic antibodies (p-ANCAs) in autoimmune hepatitis (AIH). This study confirms HMG1 and HMG2 as significant antigens in AIH patients with p-ANCAs.
Area Of Science
- Immunology
- Hepatology
- Autoimmunity
Background
- High mobility group (HMG) proteins HMG1 and HMG2 are novel antigens for perinuclear anti-neutrophil cytoplasmic antibodies (p-ANCAs).
- Previous studies reported anti-HMG1 and anti-HMG2 antibodies in ulcerative colitis patients.
Purpose Of The Study
- To determine if HMG1 and HMG2 are target antigens for p-ANCAs in autoimmune hepatitis (AIH).
Main Methods
- Serum samples from patients with AIH, primary biliary cirrhosis (PBC), and chronic hepatitis B and C were analyzed.
- Anti-neutrophil cytoplasmic antibodies (ANCAs) were detected by indirect immunofluorescence (IIF).
- Anti-HMG1 and anti-HMG2 antibodies were quantified using enzyme-linked immunosorbent assay (ELISA).
Main Results
- p-ANCAs were found in 89% of AIH patients.
- Anti-HMG1 and/or anti-HMG2 antibodies were detected in 89% of AIH patients.
- In AIH, 96% of p-ANCA positive patients had anti-HMG1/HMG2 antibodies, and IIF staining decreased after HMG1/HMG2 preincubation.
Conclusions
- High mobility group (HMG) proteins HMG1 and HMG2 are significant target antigens of p-ANCA in autoimmune hepatitis (AIH).
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