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DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)
Published on: July 15, 2011
Amplified-fragment length polymorphism analysis versus macro-restriction fragment analysis for molecular typing of
J van Eldere1, P Janssen, A Hoefnagels-Schuermans
1Department of Microbiology and Immunology, Infectious Diseases Research Group, Rega Institute for Medical Research, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium. johan.vaneldere@rega.kuleuven.ac.be
Abstract:
Forty-eight pneumococci were genotyped by on-line laser fluorescence amplified-fragment length polymorphism (AFLP) and pulsed-field gel electrophoresis (PFGE) analysis of chromosomal restriction fragments. Overall, the data generated by the two methods corresponded well. However, with AFLP, clusters were delineated at a higher similarity level, and isolate differentiation was more pronounced. AFLP and PFGE were equally efficient for assessing intraserotype diversity. We conclude that AFLP is a useful alternative to PFGE.
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