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Evaluation of capillary electrophoretic techniques towards systematic toxicological analysis.

C M Boone1, J P Franke, R A de Zeeuw

  • 1Department of Analytical Chemistry and Toxicology, State University of Groningen, The Netherlands. c.boone@farm.rug.nl

Journal of Chromatography. A
|May 18, 1999
PubMed
Summary

Two capillary electrophoresis (CE) methods, capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC), show good identification power for systematic toxicological analysis (STA). Combining these methods enhances their utility in identifying substances.

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Area of Science:

  • Analytical Chemistry
  • Forensic Chemistry

Background:

  • Systematic toxicological analysis (STA) requires robust identification methods.
  • Capillary electrophoresis (CE) offers potential for STA due to its separation capabilities.

Purpose of the Study:

  • To evaluate capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) for STA.
  • To assess the identification power (IP) of CE methods for barbiturates.

Main Methods:

  • Analysis of 25 barbiturates using CZE and MEKC.
  • Utilized specific buffer systems and an electric field strength of 52.6 kV/m.
  • Introduced corrected effective mobility to improve reproducibility of migration times.

Main Results:

  • Inter-day reproducibilities for corrected effective mobility were <0.6% (CZE) and <0.5% (MEKC).

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  • CE methods demonstrated good discriminating power and mean list length for identification.
  • Low correlation was observed between CE and chromatographic techniques (GC, HPLC).

    • Conclusions:

    • CE methods, CZE and MEKC, possess significant identification power for STA.
    • Combining CE techniques with low inter-method correlation enhances their application in toxicological analysis.