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Related Experiment Videos

Evaluating virus-transformed cell tumorigenicity.

A M Lewis1, D W Alling, S M Banks

  • 1Viral Pathogenesis Section, Laboratory of Immunopathology National Institutes of Health, Bethesda, MD 20892, USA.

Journal of Virological Methods
|May 18, 1999
PubMed
Summary

Adenovirus (Ad) 12-transformed mouse cells exhibit stable tumorigenicity traits, independent of cell immortalization. These traits, influencing tumor formation and latency, can be modulated by the host

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Area of Science:

  • Oncology
  • Virology
  • Cell Biology

Background:

  • Adenovirus (Ad) transformation of mouse cells is a model for studying tumorigenicity.
  • Understanding the factors influencing tumor development is crucial for cancer research.

Purpose of the Study:

  • To quantitatively evaluate the tumorigenicity of Ad12-transformed mouse cells.
  • To identify traits that determine tumor formation and latency.
  • To assess the influence of host immunity on tumorigenicity.

Main Methods:

  • Analyzing the relationship between tumor cell dose, tumor incidence, and tumor latency.
  • Serial passage of transformed cells in tissue culture for 52 weeks.
  • Verification using mouse cells transformed by Ad5 and SV40.

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Main Results:

  • Tumorigenicity dose-response endpoints remained stable during serial passage.
  • Two distinct traits influence tumor formation threshold and latency period.
  • These traits are independent of cell immortalization and affected by host immunological status.

Conclusions:

  • A reliable quantitative method was developed to evaluate transformed cell tumorigenicity.
  • Endogenous traits influencing tumorigenicity can be assessed and potentially manipulated.
  • Further research is needed to determine the underlying biological and molecular mechanisms.