Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cooling and freezing damage platelet membrane integrity.

T J Reid1, V F LaRussa, G Esteban

  • 1Division of Medicine, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA.

Cryobiology
|May 18, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Colorectal cancer T stage, size at diagnosis, and presentation 2017 to 2023: an analysis using Veterans Affairs data.

Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract·2026
Same author

Impact of single-use waste reduction initiatives using citizen science data: Western Australia as a case study.

Marine pollution bulletin·2025
Same author

A qualitative exploration of women's experiences of food insecurity around pregnancy aligned with the socio-ecological model.

Appetite·2025
Same author

A service evaluation following the implementation of computer guided consultation software to support primary care reviews for chronic obstructive pulmonary disease.

NPJ primary care respiratory medicine·2025
Same author

'It's especially good just to know that you're not the only one': a qualitative study exploring experiences with online peer support programmes for the Fragile X community.

Journal of intellectual disability research : JIDR·2024
Same author

Simultaneous implementation of unrelated tumour sites on the MR Linac: A review of the commissioning process from a radiographer perspective and lessons learned.

Journal of medical imaging and radiation sciences·2024

Platelet storage at 4°C causes damage, but adding EGTA/AM-CytoB offers some protection. Freezing platelets with dimethyl sulfoxide (Me2SO) best preserves membrane integrity and function.

Area of Science:

  • Biotechnology and Biomedical Engineering
  • Cellular and Molecular Biology
  • Hematology

Background:

  • Platelet cooling induces cytoskeletal changes and membrane lipid phase transitions, leading to irreversible cellular damage.
  • Existing methods to protect platelets from cooling injury, such as using calcium chelators and microfilament stabilizers, have shown limited success.
  • Understanding membrane protection mechanisms is crucial for improving platelet storage and transfusion outcomes.

Purpose of the Study:

  • To investigate the efficacy of specific agents in preserving platelet membrane integrity and function during cooling and freezing.
  • To evaluate the protective effects of a combination of EGTA/AM-CytoB against cold-induced platelet injury.
  • To assess the cryoprotective potential of dimethyl sulfoxide (Me2SO) and glycerol against freezing-induced platelet damage.

Related Experiment Videos

Main Methods:

  • Platelets were cooled to 4°C for up to 21 days with or without EGTA/AM-CytoB, and their membrane integrity was assessed via fluorescein release.
  • Platelets were frozen to -70°C with or without Me2SO or glycerol, followed by rapid thawing, and membrane integrity was measured by fluorescein release.
  • Platelet function was evaluated using ristocetin-induced agglutination, thrombin/ADP-induced aggregation, platelet-induced clot retraction (PICR), and P-selectin expression.

Main Results:

  • Cooling platelets with EGTA/AM-CytoB showed a gradual loss of membrane integrity, peaking at day 7, preceding complete functional loss.
  • Platelets stored at 4°C without protective agents lost membrane integrity and function within 1 day.
  • Freezing platelets with Me2SO resulted in significantly less fluorescein release and better preserved function compared to other cryoprotectants.

Conclusions:

  • Platelet membrane integrity loss during cooling is linked to cytoskeletal rearrangements and precedes functional impairment.
  • Dimethyl sulfoxide (Me2SO) effectively protects platelet membranes from freezing injury, preserving cellular function.
  • These findings offer insights into optimizing platelet storage conditions and may be broadly applicable to platelet preservation studies.