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Related Experiment Videos

Evolutionarily conserved, alternative splicing of reelin during brain development.

C Lambert de Rouvroit1, B Bernier, I Royaux

  • 1Neurobiology Unit, University of Namur School of Medicine, Namur, B-5000, Belgium.

Experimental Neurology
|May 18, 1999
PubMed
Summary

Researchers discovered two new forms of the reelin protein in the brain, generated by alternative splicing. These findings suggest novel functions for reelin in brain development and evolution.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • Reelin is crucial for brain development and is implicated in the reeler phenotype.
  • Reelin is typically secreted by Cajal-Retzius cells and acts on target neurons via the extracellular matrix, requiring the Dab1 gene product.
  • Expression patterns in olfactory bulb, retina, and spinal cord suggest diverse reelin functions.

Purpose of the Study:

  • To investigate the uncertainties surrounding the relationship between reelin and the reeler phenotype.
  • To identify novel splicing events and protein variants of reelin.
  • To explore the functional significance of alternative reelin forms.

Main Methods:

  • Analysis of reelin gene transcripts using molecular biology techniques.

Related Experiment Videos

  • Identification and characterization of alternative splicing events in the 3' region of reelin mRNA.
  • Comparative analysis of reelin splicing across different species (mouse, rat, human).

    • Main Results:

    • Two conserved alternative splicing events in the 3' part of the reelin transcript were identified.
    • A brain-specific 6-nucleotide microexon is skipped in approximately 10% of reelin RNA.
    • Alternative polyadenylation leads to a truncated reelin protein lacking a highly basic terminal stretch in 10-25% of mRNA, with reduced expression in retina and spinal cord.

    Conclusions:

    • The identified alternative splicing events generate functionally distinct reelin protein forms.
    • These alternative reelin forms are evolutionarily conserved in mammals, indicating functional importance.
    • The study expands our understanding of reelin's role in brain development and suggests potential new functions.