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Structural parameters from 19F homonuclear dipolar couplings, obtained by multipulse solid-state NMR on static and

S L Grage1, A S Ulrich

  • 1Institut für Molekularbiologie, Friedrich-Schiller-Universität Jena, Winzerlaer Str. 10, Jena, 07745, Germany.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|May 18, 1999
PubMed
Summary

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Fluorine-19 (19F) Nuclear Magnetic Resonance (NMR) enables precise determination of local molecular structure by measuring dipolar couplings. This technique overcomes limitations of other NMR probes, offering new insights into molecular dynamics and orientation in biological membranes.

Area of Science:

  • Solid-state Nuclear Magnetic Resonance (NMR) spectroscopy.
  • Biophysical chemistry.
  • Structural biology.

Background:

  • Solid-state NMR of dipolar couplings reveals local macromolecular structure.
  • Current probes (2H, 13C, 15N) have low sensitivity and limited distance range.
  • 1H NMR is sensitive but can perturb the system.

Purpose of the Study:

  • To introduce and validate Fluorine-19 (19F) as a superior NMR probe for measuring dipolar couplings.
  • To extend the distance range and sensitivity limitations of existing NMR methods.
  • To determine internuclear distances and angular orientation of molecules in membranes.

Main Methods:

  • Adaptation of the Carr-Purcell-Meiboom-Gill (CPMG) multipulse sequence for 19F NMR.
  • Measurement of homonuclear dipolar couplings between two 19F labels in static lipid bilayers.

Related Experiment Videos

  • Utilizing 19F-background-free model systems (lipids and sterols) at 470 MHz.

    • Main Results:

    • Resolved weak homonuclear 19F-19F dipolar couplings (down to 100 Hz) without 1H decoupling.
    • Determined order parameters for anisotropic lipid motion in liquid crystalline bilayers.
    • Extracted angular information from dipolar anisotropy in oriented membranes.

    Conclusions:

    • 19F NMR is a sensitive and effective probe for solid-state NMR structural studies.
    • The technique allows for precise determination of internuclear distances and molecular orientation.
    • This method enhances the study of molecular dynamics and structure in biological membranes.