Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

An IgA-binding peptide derived from a streptococcal surface protein.

E Johnsson¹, T Areschoug, J Mestecky

¹Department of Laboratory Medicine, Lund University, Sölvegatan 23, S-223 62 Lund, Sweden.

The Journal of Biological Chemistry

|May 18, 1999

Summary

Researchers created a synthetic peptide from Streptococcus pyogenes protein Sir22 that specifically binds human immunoglobulin A (IgA). This IgA-binding peptide domain retains its function in isolation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Impact of lymphadenectomy and lymphoedema on health-related quality of life 1 year after surgery for endometrial cancer. A prospective longitudinal multicentre study.

BJOG : an international journal of obstetrics and gynaecology·2021

Same author

Validation of instruments for the assessment of dysphagia due to malignancy of the esophagus.

Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus·2019

Same author

Effect of high pressure treatment on the color of fresh and processed meats: A review.

Critical reviews in food science and nutrition·2017

Same author

One-year efficacy and safety of saxagliptin add-on in patients receiving dapagliflozin and metformin.

Diabetes, obesity & metabolism·2016

Same author

Efficacy and safety of triple therapy with dapagliflozin add-on to saxagliptin plus metformin over 52 weeks in patients with type 2 diabetes.

Diabetes, obesity & metabolism·2016

Same author

Long-term maintenance of efficacy of dapagliflozin in patients with type 2 diabetes mellitus and cardiovascular disease.

Diabetes, obesity & metabolism·2016

Area of Science:

Microbiology
Immunology
Protein Chemistry

Background:

Streptococcus pyogenes expresses surface proteins binding the Fc region of human immunoglobulin A (IgA).
Studying these proteins is challenging due to their size and non-specific binding to other human plasma proteins.
Sir22 is a known IgA-binding protein from Streptococcus pyogenes.

Purpose of the Study:

To characterize a specific IgA-binding domain from Streptococcus pyogenes protein Sir22.
To develop a smaller, more specific IgA-binding molecule for research.

Main Methods:

Synthesis of a 50-residue peptide derived from Sir22.
Binding assays using serum IgA, secretory IgA, and various human plasma proteins.
Analysis of peptide folding, immobilization, and refolding properties.

Related Experiment Videos

Main Results:

The synthetic peptide specifically binds human IgA, including serum and secretory IgA across both subclasses.
The peptide does not bind other human plasma proteins known to interact with Sir22.
The peptide demonstrates correct folding in solution and when immobilized, and readily renatures after denaturation.

Conclusions:

The 50-residue peptide represents a functional IgA-binding domain of Sir22.
This isolated peptide domain exhibits high specificity for human IgA.
This is the first report of an IgA-binding domain retaining its properties in an isolated form.