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Crystallization and preliminary X-ray diffraction studies of piratoxin III, a D-49 phospholipase A2 from the venom of
W H Lee1, M H Toyama, A M Soares
1Laboratório Nacional de Luz Síncrotron, Caixa Postal 6192, CEP 13083-970, Campinas SP, Brazil.
Abstract:
Piratoxin III (PrTX-III) is a phospholipase A2 (PLA2, E.C. 3.1.1.4, phosphatide sn-2 acylhydrolase) isolated from Bothrops pirajai. Crystals of PrTX-III were obtained using the vapour-diffusion technique and X-ray diffraction data have been collected to 2.7 A resolution. The enzyme was crystallized in the space group C2 with unit-cell parameters a = 60.88, b = 100.75, c = 48.19 A, beta = 123. 89 degrees. A molecular-replacement solution of the structure has been found using bothropstoxin I from the venom of B. jararacussu as a search model.