Expression and localization of histamine H2 receptor messenger RNA in human nasal mucosa
Summary
This summary is machine-generated.Histamine H2 receptor (H2R) mRNA expression is significantly increased in allergic rhinitis patients, suggesting a role for H2R in this condition.
Area Of Science
- Immunology
- Otorhinolaryngology
- Molecular Biology
Background
- Histamine is a key mediator in allergic rhinitis.
- Histamine H2 receptor (H2R) stimulation influences histamine release and mucus secretion.
- These functions suggest a potential role for H2R in allergic rhinitis pathogenesis.
Purpose Of The Study
- To investigate if Histamine H2 receptor (H2R) mRNA expression is elevated in allergic rhinitis patients compared to healthy individuals.
- To determine the specific location of H2R mRNA within the human nasal mucosa.
Main Methods
- RNA extraction from inferior turbinate mucosa scrapings of allergic rhinitis patients and normal subjects.
- Reverse transcription-polymerase chain reaction (RT-PCR) to amplify H2R and beta-actin mRNA.
- In situ hybridization using a digoxigenin-labeled H2R cRNA probe on nasal turbinate specimens.
Main Results
- H2R/beta-actin mRNA ratios were significantly higher in allergic rhinitis patients (0.35 +/- 0.20) than in normal subjects (0.12 +/- 0.16) (P <.01).
- H2R mRNA was detected in the epithelium, serous cells, and mucous cells of the nasal mucosa.
Conclusions
- H2R mRNA is present in the nasal epithelium and submucosal glands.
- Elevated H2R expression may contribute to the pathophysiology of allergic rhinitis.
View abstract on PubMed