Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hypoperfusion-induced contractile failure does not require changes in cardiac energetics.

K W Saupe1, F R Eberli, J S Ingwall

  • 1Cardiac Muscle Research Laboratory, Boston University School of Medicine, Boston, Massachusetts 02118, USA.

The American Journal of Physiology
|May 18, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improved two-year outcomes after drug-eluting versus bare-metal stent implantation in women and men with large coronary arteries: importance of vessel size.

International journal of cardiology·2013
Same author

The impact of statin treatment on presentation mode and early outcomes in acute coronary syndromes.

Cardiology·2007
Same author

Energetics of the failing heart: new insights using genetic modification in the mouse.

Archives des maladies du coeur et des vaisseaux·2006
Same author

Images in cardiology. No reflow during carotid stenting.

Heart (British Cardiac Society)·2006
Same author

[Mechanical complications of acute myocardial infarction].

Zeitschrift fur Kardiologie·2004
Same author

[Valve disease: what the general practicioner needs to know].

Praxis·2004

Reduced blood flow to the heart immediately impairs function, but not due to energy depletion. Non-energetic factors, not changes in cardiac energetics, dominate initial contractile dysfunction during hypoperfusion.

Area of Science:

  • Cardiology
  • Biochemistry
  • Physiology

Background:

  • Decreased coronary perfusion leads to rapid loss of heart muscle function.
  • Cardiac energetics (energy production and use) are suspected to cause this dysfunction.
  • The precise cause of this immediate contractile dysfunction remains unclear.

Purpose of the Study:

  • To investigate if changes in cardiac energetics precede the decline in contractile function.
  • To determine the causal relationship between energy metabolism and heart muscle function during reduced blood flow.
  • To identify the primary drivers of contractile dysfunction during myocardial hypoperfusion.

Main Methods:

  • Utilized 14 isolated rat hearts for the study.
  • Gradually reduced coronary perfusion in a controlled manner.

Related Experiment Videos

  • Employed 31P NMR spectroscopy to measure key energy metabolites (ATP, PCr, Pi, ADP), pH, and free energy from ATP hydrolysis (|DeltaGATP|).

    • Main Results:

    • No significant changes in ATP, PCr, ADP, or pH were observed until several minutes after systolic pressure decreased.
    • Even with a significant drop in developed pressure, changes in Pi, pH, and |DeltaGATP| were minimal.
    • The rate of high-energy phosphate transfer did not decrease sufficiently to account for the observed loss of contractile function.

    Conclusions:

    • Non-energetic factors are the primary cause of initial systolic dysfunction during decreased myocardial perfusion.
    • Cardiac energetics do not appear to be the dominant factor in the immediate contractile dysfunction.
    • The study challenges the long-held hypothesis linking ischemia-induced changes in cardiac energetics to immediate contractile dysfunction.