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Related Experiment Videos

A new sample-processing unit for the fluorescent microsphere method.

S Raab1, E Thein, A G Harris

  • 1Institute for Surgical Research, University of Munich, 81366 Munich, Germany.

The American Journal of Physiology
|May 18, 1999
PubMed
Summary
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This study introduces a new filtration device to simplify fluorescent-labeled microsphere (FM) recovery from tissue samples, preventing FM loss and improving regional blood flow measurements.

Area of Science:

  • Biomedical Engineering
  • Physiology
  • Analytical Chemistry

Background:

  • Fluorescent-labeled microspheres (FM) offer an alternative to radioactive methods for regional blood flow measurement.
  • Current FM methods require extensive tissue processing, leading to potential microsphere loss.
  • This loss can compromise the accuracy of blood flow quantification.

Purpose of the Study:

  • To develop an improved filtration device and protocol for processing tissue samples in FM-based blood flow analysis.
  • To streamline the recovery of fluorescent-labeled microspheres (FM) from various organs.
  • To minimize microsphere loss and enhance the standardization of the procedure.

Main Methods:

  • A novel sample-processing unit (SPU) featuring a single-tube filtration device with a polyamide wire mesh was developed.

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  • The protocol for processing diverse organ tissues (heart, kidney, liver, spleen, intestine, muscle, bone, lung, brain) was significantly modified and shortened.
  • The SPU enabled direct filtration of blood reference samples, eliminating the need for prior digestion.

    • Main Results:

    • The developed SPU and protocol successfully prevented the loss of 15-micrometer FM during tissue processing.
    • The modified procedure was significantly faster and more labor-efficient compared to conventional methods.
    • The entire process was rendered highly standardized across different organ types.

    Conclusions:

    • The novel filtration device and streamlined protocol effectively address the limitations of existing FM methods for regional blood flow measurement.
    • This innovation enhances the accuracy and efficiency of blood flow quantification by ensuring complete FM recovery.
    • The standardized procedure facilitates more reliable and reproducible physiological research.